Figure A. Alexa 488 conjugated mouse anti human CD56 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. Alexa 488 conjugated mouse anti human CD56 and Alexa 647 conjugated mouse anti human CD16 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .

Mouse anti Human CD16 antibody, clone LNK16 used as a blocking agent to assess the effect of Fc?Rs on heparin binding protein (HBP) release by ELISA.

Image caption:

Antibodies against Fc?RII inhibit HBP release.
Whole blood (diluted 1?10) was incubated with antibodies against different Fc?Rs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.


From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008) Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD56 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD56 and Amethyst Orange conjugated Mouse anti Human CD16 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer