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CD19 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225849

Mouse anti Human CD19 antibody, clone LT19 recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and… More details

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Product Overview

Product Name
CD19, Monoclonal Antibody
Full Product Name
Mouse Anti Human CD19: Amethyst Orange
Product Gene Name
anti-CD19 antibody
Product Synonym Names
CD19 antibody, LT19
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LT19
Host
Mouse
Species Reactivity
Human
Form/Format
Purified IgG conjugated to Amethyst Orange - Liquid
Purity/Purification
Purified IgG from ascites prepared by precipitation and ion exchange chromatography.
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
OMIM
107265
3D Structure
ModBase 3D Structure for P15391
Preservative Stabilizers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Buffer Solution
Phosphate Buffered Saline
Applications Tested/Suitable for anti-CD19 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD19 antibody
FC/FACS: Min. Dilution: Neat
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul

Testing Data of anti-CD19 antibody

Figure A. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated mouse anti human CD19 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock
Mouse Anti-CD19 Monoclonal Antibody – Cat# MBS225849

Testing Data of anti-CD19 antibody

Figure A. RPE conjugated mouse anti human CD40 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD40 and FITC conjugated mouse anti human CD19 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .
Mouse Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS225849

Testing Data of anti-CD19 antibody

Figure A. Alexa488 conjugated mouse anti human CD4 and Pacific Blue conjugated Mouse IgG1 isotype control . Figure B. Alexa488 conjugated mouse anti human CD4 and Pacific Blue conjugated mouse anti human CD19 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .
Mouse Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS225849

Testing Data of anti-CD19 antibody

Figure A. FITC conjugated Rat anti Human CD18 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Rat anti Human CD18 and RPE conjugated Mouse anti Human CD19 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .
Mouse Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS225849

Testing Data of anti-CD19 antibody

Figure A. Pacific Blue conjugated mouse anti human HLA ABC and A488 conjugated mouse IgG2a isotype control . Figure B. Pacific Blue conjugated mouse anti human HLA ABC and A488 conjugated mouse anti Human CD19 . All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer
Mouse Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS225849

Testing Data of anti-CD19 antibody

Figure A. PE conjugated Mouse anti Human CD22 and FITC conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD22 and FITC conjugated Mouse anti Human CD19 . All experiments performed on human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS225849

Testing Data of anti-CD19 antibody

Figure A. PE conjugated Mouse anti Human CD20 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD20 and Amethyst Orange conjugated Mouse anti Human CD19 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Mouse Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS225849

Other Notes

Small volumes of anti-CD19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD19 purchase

MBS225849 is an antibody targeting CD19. Also known as Mouse Anti Human CD19: Amethyst Orange. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD19. It may not necessarily be applicable to this product.

NCBI GI #
296010921
NCBI GeneID
930
NCBI Accession #
NP_001171569.1
UniProt Primary Accession #
P15391
UniProt Related Accession #
P15391
GenBank Nucleotide #
NP_001171569.1
NCBI Official Full Name
B-lymphocyte antigen CD19 isoform 1
NCBI Official Synonym Full Names
CD19 molecule
NCBI Official Symbol
CD19
NCBI Official Synonym Symbols
B4; CVID3
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Synonym Protein Names
B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12
Protein Family
B-lymphocyte antigen
UniProt Gene Name
CD19
UniProt Entry Name
CD19_HUMAN
NCBI Protein Information
B-lymphocyte antigen CD19

NCBI Summary for CD19

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD19

CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: protein complex; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: receptor signaling protein activity

Biological Process: epidermal growth factor receptor signaling pathway; B cell receptor signaling pathway; regulation of immune response; cell surface receptor linked signal transduction; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; cellular defense response; innate immune response; positive regulation of release of sequestered calcium ion into cytosol

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 3
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Research Articles on CD19

1. CD19 antigen has a role in duration of CAR T engraftment in leukemia patients treated with CD19 CAR+ T cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD19 antibody

 Reference Product  PubMed Publications
 CD22 antibody  >563 publications with CD19 and CD22
 CD79A antibody  >134 publications with CD19 and CD79A
 CD79B antibody  >99 publications with CD19 and CD79B
 CD81 antibody  >89 publications with CD19 and CD81
 CD9 antibody  >78 publications with CD19 and CD9
 CR2 antibody  >75 publications with CD19 and CR2
 LYN antibody  >55 publications with CD19 and LYN
 SYK antibody  >40 publications with CD19 and SYK
 BTK antibody  >36 publications with CD19 and BTK
 VAV1 antibody  >13 publications with CD19 and VAV1

Pathways associated with anti-CD19 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram

Diseases associated with anti-CD19 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2854 publications with CD19 and Neoplasms
 Lymphoma Antibodies  >1667 publications with CD19 and Lymphoma
 Cardiovascular Diseases Antibodies  >400 publications with CD19 and Cardiovascular Diseases
 Inflammation Antibodies  >301 publications with CD19 and Inflammation
 Necrosis Antibodies  >293 publications with CD19 and Necrosis
 Precursor B-Cell Lymphoblastic Leukemia-Lymphoma Antibodies  >251 publications with CD19 and Precursor B-Cell Lymphoblastic Leukemia-Lymphoma
 Arthritis, Rheumatoid Antibodies  >177 publications with CD19 and Arthritis, Rheumatoid
 Liver Diseases Antibodies  >147 publications with CD19 and Liver Diseases
 Kidney Diseases Antibodies  >126 publications with CD19 and Kidney Diseases
 Drug Toxicity Antibodies  >125 publications with CD19 and Drug Toxicity

Organs/Tissues associated with anti-CD19 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5630 publications with CD19 and Blood
 Bone Marrow Antibodies  >1613 publications with CD19 and Bone Marrow
 Spleen Antibodies  >509 publications with CD19 and Spleen
 Lymph Node Antibodies  >439 publications with CD19 and Lymph Node
 Lymph Antibodies  >392 publications with CD19 and Lymph
 Lung Antibodies  >196 publications with CD19 and Lung
 Thymus Antibodies  >167 publications with CD19 and Thymus
 Brain Antibodies  >115 publications with CD19 and Brain
 Tonsil Antibodies  >112 publications with CD19 and Tonsil
 Intestine Antibodies  >95 publications with CD19 and Intestine
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