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CD33 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225852

Mouse anti Human CD33 antibody, clone WM53 recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin.Mouse anti Human CD33 antibody,… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Product Name
CD33, Monoclonal Antibody
Full Product Name
Mouse Anti Human CD33: Amethyst Orange
Product Gene Name
anti-CD33 antibody
Product Synonym Names
CD33 antibody, WM53
Clonality
Monoclonal
Isotype
IgG1
Clone Number
WM53
Host
Mouse
Species Reactivity
Human
Form/Format
Purified IgG conjugated to Amethyst Orange - Liquid
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
OMIM
159590
3D Structure
ModBase 3D Structure for P20138
Immunogen
Human AML cells
Preservative Stabilizers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Buffer Solution
Phosphate Buffered Saline
Applications Tested/Suitable for anti-CD33 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD33 antibody
FC/FACS: Max. Dilution: Neat
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Testing Data of anti-CD33 antibody

FITC conjugated Mouse anti Human CD14 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD14 and Amethyst Orange conjugated Mouse anti Human CD33 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Mouse Anti-CD33 Monoclonal Antibody – Cat# MBS225852

Testing Data of anti-CD33 antibody

Figure A. FITC conjugated mouse anti human CD14 and Alexa647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD14 and Alexa647 conjugated mouse anti human CD33 . All experiments performed on red cell lysed Human peripheral blood gated on myeloid cells in the presence of Human SeroBlock .
Mouse Anti-CD33 Monoclonal Antibody, Testing Data – Cat# MBS225852

Testing Data of anti-CD33 antibody

Mouse anti Human CD33 antibody, clone WM53 used to evaluate CD33 expression on blood derived NK cells by flow cytometry.

Image caption:

CD33 expression and function in primary human NK cells. (a) Blood NK cells (CD3-CD56+ lymphocytes in region R1) and (b, c) quiescent primary human NK cells were phenotyped by flow cytometry. Blood CD33+ NK cells were mainly CD56bright (R2 region, dark dots), CD16-, CD57-, NKG2A+, and NKG2D+. The figure shows a representative blood sample out of the four tested.


From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.

CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019 Apr 15;2019:6032141.

doi: 10.1155/2019/6032141.

This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse Anti-CD33 Monoclonal Antibody, Testing Data – Cat# MBS225852

Testing Data of anti-CD33 antibody

Mouse anti Human CD33 antibody, clone WM53 used to evaluate CD33 expression on blood derived NK cells using immunoprecipitation.

Image caption:

CD33 expression and function in primary human NK cells. (d) CD33 coprecipitated SHP-2 phosphatase only in quiescent primary human NK cell lysates. The figure shows a representative experiment out of two with similar results.


From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.
CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019 Apr 15;2019:6032141.
doi: 10.1155/2019/6032141.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse Anti-CD33 Monoclonal Antibody, Testing Data – Cat# MBS225852

Testing Data of anti-CD33 antibody

Mouse anti Human CD33 antibody, clone WM53 used to evaluate CD33 expression on blood derived quiescent NK cells by flow cytometry.
Image caption:
CD33 expression and function in primary human NK cells.CD33+ (WM53+ HIM3-4-) quiescent NK cells (R1 region, dark dots) were CD57-, NKG2D+, and partially expressed NKp46 and NKG2A antigens.


From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.
CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019 Apr 15;2019:6032141.

doi: 10.1155/2019/6032141.

This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse Anti-CD33 Monoclonal Antibody, Testing Data – Cat# MBS225852

Other Notes

Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD33 purchase

MBS225852 is an antibody targeting CD33. Also known as Mouse Anti Human CD33: Amethyst Orange. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product.

NCBI GI #
116241290
NCBI GeneID
945
NCBI Accession #
P20138.2
UniProt Primary Accession #
P20138
UniProt Related Accession #
P20138
NCBI Official Full Name
Myeloid cell surface antigen CD33
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
UniProt Protein Name
Myeloid cell surface antigen CD33
UniProt Synonym Protein Names
Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33
Protein Family
Myeloid cell surface antigen
UniProt Gene Name
CD33
UniProt Synonym Gene Names
SIGLEC3; Siglec-3
UniProt Entry Name
CD33_HUMAN
NCBI Protein Information
myeloid cell surface antigen CD33

UniProt Comments for CD33

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity; carbohydrate binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion
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Research Articles on CD33

1. This review summarizes the recently advanced biology of CD33 and its association with Alzheimer's disease. [Review Article]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD33 antibody

 Reference Product  PubMed Publications
 CD34 antibody  >1065 publications with CD33 and CD34
 SRC antibody  >20 publications with CD33 and SRC
 PTPN11 antibody  >11 publications with CD33 and PTPN11
 PTPN6 antibody  >7 publications with CD33 and PTPN6
 CBL antibody  >3 publications with CD33 and CBL
 UBC antibody  >3 publications with CD33 and UBC
 PVR antibody  >1 publications with CD33 and PVR

Pathways associated with anti-CD33 antibody

 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD33 antibody

 Disease Name  Pubmed Publications
 Leukemia, Myeloid Antibodies  >1323 publications with CD33 and Leukemia, Myeloid
 Leukemia, Myeloid, Acute Antibodies  >1160 publications with CD33 and Leukemia, Myeloid, Acute
 Necrosis Antibodies  >92 publications with CD33 and Necrosis
 Death Antibodies  >78 publications with CD33 and Death
 Cardiovascular Diseases Antibodies  >77 publications with CD33 and Cardiovascular Diseases
 Inflammation Antibodies  >67 publications with CD33 and Inflammation
 Respiratory Tract Diseases Antibodies  >44 publications with CD33 and Respiratory Tract Diseases
 Brain Diseases Antibodies  >40 publications with CD33 and Brain Diseases
 Breast Neoplasms Antibodies  >39 publications with CD33 and Breast Neoplasms
 Leukocytosis Antibodies  >32 publications with CD33 and Leukocytosis

Organs/Tissues associated with anti-CD33 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1414 publications with CD33 and Blood
 Bone Antibodies  >982 publications with CD33 and Bone
 Bone Marrow Antibodies  >971 publications with CD33 and Bone Marrow
 Liver Antibodies  >85 publications with CD33 and Liver
 Lymph Node Antibodies  >53 publications with CD33 and Lymph Node
 Lung Antibodies  >50 publications with CD33 and Lung
 Brain Antibodies  >31 publications with CD33 and Brain
 Thymus Antibodies  >29 publications with CD33 and Thymus
 Kidney Antibodies  >19 publications with CD33 and Kidney
 Prostate Antibodies  >10 publications with CD33 and Prostate
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