Rat anti Mouse CD45R antibody, clone RA3-6B2 used for the enumeration of B cells in the intestinal mucosa by immunofluorescence.

Image caption:

Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 ug/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistochemistry. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.


From: Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F (2009)

Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation.

PLoS ONE 4(12): e8291.

This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Figure A. Alexa647 conjugated hamster anti mouse CD79b and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa647 conjugated hamster anti mouse CD79b and RPE conjugated rat anti mouse CD45R . All experiments performed on murine bone marrow in the presence of murine SeroBlock .

Figure A. RPE conjugated mouse anti mouse CD28 and Alexa647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated mouse anti mouse CD28 and Alexa647 conjugated rat anti mouse CD45 . All experiments performed on murine splenocytes in the presence of murine SeroBlock .

Figure A. RPE conjugated Rat anti Mouse CD36 and A488 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD36 and A488 conjugated Rat anti Mouse CD45R . All experiments were performed on mouse splenocytes in the presence of murine SeroBlock

Rat anti Mouse CD45R antibody, clone RA3-6B2 used for the identification of marginal zone B cells in mouse spleen by immunofluorescence on cryostat sections.

Image caption:

Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.


From: Zhou Z, Niu H, Zheng YY, Morel L.

Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.

BMC Immunol. 2011 Jan 20;12:7.

This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rat anti MouseCD45R antibody, clone RA3-6B2 used for the demonstration of B cells in murine spinal column by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.

Image caption:

Representative images of key immunohistochemical markers run on a longitudinally sectioned, decalcified spinal column from an EAE mouse with a clinical EAE score of 4.
(A) HE, (B) B220 immunohistochemistry for (B) cells, (C) F4/80 immunohistochemistry for macrophages (arrow indicates area highlighted in inset, inset bar = 20 ?m), (D) CD3 immunohistochemistry for T cells. Bars = 200 ?m.


From: Gibson-Corley KN, Boyden AW, Leidinger MR, Lambertz AM, Ofori-Amanfo G, Naumann PW, Goeken JA, Karandikar NJ.

A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.

PeerJ. 2016 Jan 26;4:e1600.

This image is from an open access article distributed under the terms of the Creative Commons Attribution License.