Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD8 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection system . Low power
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System . Medium power
Mouse anti Human CD8 antibody, clone LT8 used for the evaluation of CD8? exptession on T lymphocytes from marmoset blood, spleen and lung by flow cytometry.
Image caption:
The percentage of the total leucocyte count for various cell types identified in naive marmosets (a) cells in naïve blood, (b) in naive spleens, and (c) in naive lungs. CD8 T cells are expressed as a percentage of CD3+ cells. Bar represents the median value.
From: M. Nelson and M. Loveday, “Exploring the Innate Immunological Response of an Alternative Nonhuman Primate Model of Infectious Disease; the Common Marmoset,”
Journal of Immunology Research, vol. 2014, Article ID 913632, 8 pages, 2014.
Figure A. FITC conjugated mouse anti human CD3 and APC conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and APC conjugated mouse anti human CD8 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock
Alexafluor 700 conjugated Mouse anti Human CD8 antibody, clone LT8 used for the evaluation of CD8 expression on PBMC by flow cytometry.
Image caption:
Gating stategy for cell surface marker analysis in uncultured and cultured PBMC. Gates were applied in the numerical order shown.
From: Manivannan K, Rowan AG, Tanaka Y, Taylor GP, Bangham CRM (2016)
CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.
PLoS Pathog 12(4): e1005560.
Figure A. Alexa647 conjugated rat anti human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. Alexa647 conjugated rat anti human CD3 and RPE conjugated mouse anti human CD8 . All experiments performed on red cell lysedand permeabilized Human peripheral blood gated on leukocytes in the presence of Human SeroBlock .
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System . Medium power
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System . High power
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse anti Human CD8 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
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