LEUCOPERM Accessory Reagent

Flow cytometric analyses with monoclonal antibodies have been restricted primarily to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies.
Also excluded from flow cytometric studies were cytoplasmic localisations of well established membrane molecules such as CD3 and CD22.
LEUCOPERM reagents allow intracellular antigen analysis with the same ease as surface antigens. The only prerequisite is the availability of suitable antibody conjugates. Most commercially available monoclonal antibody conjugates can be used with LEUCOPERM reagents. Some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time may have to be determined experimentally for each antibody conjugate.

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2. Grundy, M. et al. (2010) The FLT3 internal tandem duplication mutation is a secondary target of the aurora B kinase inhibitor AZD1152-HQPA in acute myelogenous leukemia cells.Mol Cancer Ther. 9: 661-72.
3. Taylor, L. et al. (2010) The effect of acute hypoxia on heat shock protein 72 expression and oxidative stress in vivo.Eur J Appl Physiol. 109 (5): 849-55.
4. Myles, A. et al. (2011) Expression of Toll-like receptors 2 and 4 is increased in peripheral blood and synovial fluid monocytes of patients with enthesitis-related arthritis subtype of juvenile idiopathic arthritis.Rheumatology (Oxford). 50: 481-8.
5. Osorio, Y. et al. (2011) Identification of small molecule lead compounds for visceral leishmaniasis using a novel ex vivo splenic explant model systemPLoS Negl Trop Dis. 5:e962.

Small volumes of LEUCOPERM accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS225936 is an accessory reagent targeting LEUCOPERM.