Figure A. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat anti Dog CD4 . All experiments performed on red cell lysed canine blood gated on lymphoid cells in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.
Figure A. Alexa Fluor 647 conjugated Rat anti Canine CD5 and Alexa Fluor 488 conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Canine CD5 and Alexa Fluor 488 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on lymphocytes in the presence of 10% canine serum. Data acquired on the ZE5 Cell Analyzer.
Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Figure A. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse anti Canine CD3 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Figure A. RPE conjugated Mouse anti Canine CD5 and A647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Canine CD5 and A647 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Figure A. A488 conjugated Rat anti Canine CD4 and purified Mouse IgG1 isotype control detected with Goat anti Mouse IgG1 DyLight 649 . Figure B. A488 conjugated Rat anti Canine CD4 and purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Figure A. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b FITC isotype control . Figure B. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 PE and Rat anti Canine CD4 FITC . All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Published customer image:
Pacific Blue conjugated Mouse anti Canine CD4 antibody, clone YKIX302.9 used for evaluation of CD4 expression on canine cells by flow cytometry.
Image caption:
CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hrs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.
From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
Published customer image:
RPE conjugated Mouse anti Canine CD4 antibody, clone YKIC302.9used for the assessment of CD4 levels on canine cells by flow cytometry.Image caption:
Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.
From: From: Estrela-Lima et al.
BMC Cancer 2010 10:256.
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