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CD8 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225955

Mouse anti Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.Under reducing conditions, the antigens immunoprecipitated by Mouse anti Sheep CD8 antibody, clone… More details

$460 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD8, Monoclonal Antibody
Full Product Name
MOUSE ANTI SHEEP CD8
Product Gene Name
Product Synonym Names
CD8 antibody|38.65
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
38.65
Host
Mouse
Species Reactivity
Sheep
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
CD8
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Species Cross-Reactivity
Bovine, Goat
Immunogen
Ovine efferent lymphocytes.
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line
Carrier Free
Yes
Applications Tested/Suitable for anti-CD8 antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)-Frozen
Application Notes for anti-CD8 antibody
FC/FACS: 1/50-1/100
FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Testing Data of anti-CD8 antibody

Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine CD8:FITC .
Mouse Anti-CD8 Monoclonal Antibody – Cat# MBS225955

Product References and Citations for anti-CD8 antibody

1. Maddox, J.F. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.Immunology. 55 (4): 739-48.
2. Mackay, C.R. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.Eur J Immunol. 16 (1): 19-25.
3. Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.Cell Immunol. 110 (1): 46-55.
4. Mackay, C.R. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development.Eur J Immunol. 19 (8): 1477-83.
5. Mackay, C.R. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.J Immunol. 136 (5): 1592-9.

Other Notes

Small volumes of anti-CD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD8 purchase

MBS225955 is an antibody targeting CD8. Also known as MOUSE ANTI SHEEP CD8. Reactive in Sheep. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD8. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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