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CD197 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225958

Rat anti Mouse CD197 antibody, clone 4B12 recognizes murine C-C chemokine receptor type 7, also known as CD197, Epstein-Barr virus-induced G-protein coupled receptor 1, CCR7 or MIP-3 beta receptor. CD197 is a 354 amino acid ~43 kDa member of the G-protein… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD197, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD197
Product Gene Name
Product Synonym Names
CD197 antibody|4B12
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4B12
Host
Rat
Species Reactivity
Mouse
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
CD197
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Immunogen
CCR7 rat basophilic leukaemia (RBL)-2H3 transfectants.
Preservative Stabilizers
0.09% Sodium Azide
Fusion Partners
Spleen cells from immunised Lou/c rats were fused with cells of the mouse X63.Ag8.653 myeloma cell line
Carrier Free
Yes
Applications Tested/Suitable for anti-CD197 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes for anti-CD197 antibody
FC/FACS: 1/25-1/100
FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Testing Data of anti-CD197 antibody

Staining of mouse spleen cells probed with Rat anti Mouse CD197: Alexa Fluor 647 conjugated Rat anti Mouse CD197 antibody, clone 4B12
Rat Anti-CD197 Monoclonal Antibody – Cat# MBS225958

Product References and Citations for anti-CD197 antibody

1. Ritter, U. et al. (2004) Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.J Leukoc Biol. 76 (2): 472-6.

Other Notes

Small volumes of anti-CD197 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD197 purchase

MBS225958 is an antibody targeting CD197. Also known as RAT ANTI MOUSE CD197. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD197. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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