Rat Anti-CD31 Monoclonal Antibody – Cat# MBS225963

Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.

CD31 Antibody – Rat Monoclonal

Name: CD31 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS225963
Price: 420 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225963

Rat anti Mouse CD31 antibody, clone ER-MP12 recognizes mouse CD31, a 140 kDa cell surface glycoprotein that is expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.CD31 is also expressed on a major population of… More details

$420 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD31, Monoclonal Antibody

Full Product Name

RAT ANTI MOUSE CD31

Clonality

Monoclonal

Host

Rat

Species Reactivity

Mouse

Form/Format

Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Concentration

IgG concentration 1.0mg/ml (lot specific)

Specificity

CD31

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Immunogen

BALB/c macrophage precursor cell hybrids

Preservative Stabilizers

0.09% Sodium Azide

Fusion Partners

Cells from immunised rats were fused with the cells of the rat Y3-Ag1.2.3 myeloma cell line

Carrier Free

Yes

Applications Tested/Suitable for anti-CD31 antibody

Immunohistochemistry (IHC)-Frozen, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence

Application Notes for anti-CD31 antibody

FC/FACS: Min Dilution: 1/50 (Pack Size: 25ug, 0.25mg), 1/10 (Pack Size: 0.1mg); Max Dilution: 1/200 (Pack Size: 25ug, 0.25mg), 1/100 (Pack Size: 0.1mg)
(IHC)-Frozen: 1/10-1/100
FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product References and Citations for anti-CD31 antibody

1. Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.Eur J Immunol. 20 (1): 27-34.
2. Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.Am J Pathol. 158 (1): 131-45.
3. de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.J Immunol Methods. 217 (1-2): 27-39.
4. Revermann, M. et al. (2010) Soluble epoxide hydrolase deficiency attenuates neointima formation in the femoral cuff model of hyperlipidemic mice.Arterioscler Thromb Vasc Biol. 30: 909-14.
5. Thorp, E. et al. (2011) A reporter for tracking the UPR in vivo reveals patterns of temporal and cellular stress during atherosclerotic progression.J Lipid Res. 52 (5): 1033-8.

Other Notes

Small volumes of anti-CD31 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD31 purchase

MBS225963 is an antibody targeting CD31. Also known as RAT ANTI MOUSE CD31. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD31. It may not necessarily be applicable to this product.

Protein Family

Platelet endothelial cell adhesion molecule

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.