Bovine IFN GAMMA Elisa Kit – Cat# MBS225975

Typical assay curve using standards and reagents supplied in the Bovine IFN gamma ELISA kit

Interferon GAMMA (IFN GAMMA) Elisa Kit for Bovine

Name: Bovine IFN GAMMA Elisa Kit; Interferon GAMMA; MyBioSource
Brand: MyBioSource
SKU: MBS225975
Price: 900 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225975

The Bovine Interferon Gamma Kit is specific for bovine interferon gamma (IFN-gamma), a secreted cytokine involved in anti-viral activity and growth regulation belonging to the type II interferon family.IFN-gamma is produced by lymphocytes in response to… More details

$900 / 5x96 Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

INTERFERON GAMMA (IFN Gamma), Elisa Kit

Full Product Name

BOVINE INTERFERON GAMMA ELISA KIT

Product Gene Name

IFN Gamma elisa kit

Product Synonym Names

IFN Gamma

Species Reactivity

Bovine

Specificity

IFN GAMMA

Preparation and Storage

Prior to use store all kit components at 4 degree C. DO NOT FREEZE. Please view the handbook for further details.
Storage in frost free freezers is not recommended. This Kit contains components that are photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature components within this kit.
The contents of this kit should be stored undiluted. Should components within this kit contain a precipitate we recommend microcentrifugation before use.

Reagents in the Kit

96 well microtiter plates x 5
Microplate adhesive sealers x 5
Coating antibody - 1vial
Coating Buffer - 1 vial (5 x concentrated)
Detection antibody - Biotin conjugated - 1 vial
Streptavidin: HRP conjugate - 1 vial
TMB Substrate 1 vial
Bovine IFN gamma standard (400ng/ml - Lyophilized) 1 vial

Intended Use

Bovine Interferon gamma ELISA KIt is an ELISA kit (5 x 96 wells) designed for the quantitive determination of bovine interferon gamma (BoIFN-gamma) in bovine serum, plasma and culture supernatant.
This assay can measure BoIFN-gamma in the range 0.025-50 ng/ml in bovine serum, plasma and culture supernatant. Detailed instructions for use of the kit can be found here.
The following additional reagents and Equipment are also required for successful use of this kits BUT ARE NOT CONTAINED WITHIN THIS KIT
Wash buffer (see kit insert for recipe)
Blocking solution (or see kit insert for recipe)
Stop Solution (see kit insert for recipe)
Distilled or Deionised water
Plate reader with 450nm reading capacity
Pipettes for dispensing up to 250ul
Tubes for preparing standard and sample dilutions.

Applications Tested/Suitable for IFN Gamma elisa kit

ELISA (EIA)

Application Notes for IFN Gamma elisa kit

Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Elisa: This assay is based on a sandwich ELISA using two different mouse anti bovine IFN-gamma monoclonal antibodies and a recombinant bovine IFN-gamma as a standard.

Other Notes

Small volumes of IFN Gamma elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IFN Gamma purchase

MBS225975 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the INTERFERON GAMMA (IFN Gamma) Elisa Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFN Gamma. The ELISA analytical biochemical technique of the MBS225975 kit is based on IFN Gamma antibody-IFN Gamma antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFN Gamma antigen targets in samples. The Elisa Kit is designed to detect native, not recombinant, IFN Gamma. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IFN Gamma. It may not necessarily be applicable to this product.

Protein Family

Interferon gamma receptor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.