CD20 Antibody – Monoclonal

Monoclonal

IgG1

10F381

HEK

Human

Human IgG1 (kappa) antibody, liquid. Phosphate buffered saline
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

>98% by SDS PAGE

IgG concentration 1.0mg/ml (lot specific)

This product is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic rituximab antibody clone 10F381. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-rituximab antibodies, for example HCA186 and HCA201. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-rituximab antibody in IgG1 format as a reference standard, for example HCA201. Rituximab is a chimeric monoclonal antibody with murine variable region and human constant domains. It targets CD20, a protein found on the surface of B cells. Binding of the drug to its target triggers cell death through a number of mechanisms, including apoptosis, complement activation and cell-mediated cytotoxicity. Rituximab has been approved for the treatment of B cell malignancies, transplant rejection and autoimmune disorders.

It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed.
Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody.
Storage in frost-free freezers is not recommended.

Human lymphoblastoid cell line SB

0.02% Proclin 300