Escherichia COLI lacZ Active Protein – Cat# MBS230338

BETA Galactosidase (lacZ) Escherichia COLI Active Protein

Name: lacZ Active Protein; BETA Galactosidase; Escherichia COLI; MyBioSource
Brand: MyBioSource
SKU: MBS230338
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS230338

Escherichia coli Beta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It… More details

$580 / 5 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BETA GALACTOSIDASE (lacZ), Active Protein

Full Product Name

NATIVE ESCHERICHIA COLI BETA GALACTOSIDASE

Product Gene Name

lacZ active protein

Form/Format

Purified protein from Escherichia coli - lyophilized

Concentration

Protein content is approximately 20-25% of total weight (lot specific)

Preparation and Storage

Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the protein.
Should this product contain a precipitate we recommend microcentrifugation before use.

Molecular Weight

540 kD

3D Structure

ModBase 3D Structure for P00722

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Target Species

Bacterial

Reconstitution

Reconstitute with 1.0 ml sterile distilled water or aqueous buffer of choice. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. It is recommended that the vial is gently mixed after reconstitution.

Buffer Solution

Phosphate buffered saline

Preservative Stabilizers

Contains Sucrose

Activity

>600 U/mg protein
One unit hydrolyses 1 umole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC. For batch-specific activity, please inquire.

Applications Tested/Suitable for lacZ active protein

Functional Assays (FN)

Application Notes for lacZ active protein

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Other Notes

Small volumes of lacZ active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for lacZ purchase

MBS230338 is an active protein targeting lacZ. Also known as NATIVE ESCHERICHIA COLI BETA GALACTOSIDASE.

Additional Details

NCBI/Uniprot data below describe general gene information for lacZ. It may not necessarily be applicable to this product.

NCBI GI #

16128329

NCBI GeneID

945006

NCBI Accession #

NP_414878.1

UniProt Primary Accession #

P00722

UniProt Secondary Accession #

Q2MC80

GenBank Nucleotide #

NC_000913.3

NCBI Official Full Name

beta-D-galactosidase

NCBI Official Symbol

lacZ

NCBI Official Synonym Symbols

ECK0341; JW0335

UniProt Protein Name

Beta-galactosidase

UniProt Synonym Protein Names

Lactase

Protein Family

Beta-galactosidase

UniProt Gene Name

lacZ

UniProt Synonym Gene Names

Beta-gal

UniProt Entry Name

BGAL_ECOLI

NCBI Protein Information

beta-D-galactosidase

NCBI Summary for lacZ

Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527].

UniProt Comments for lacZ

lacZ: Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527]

Protein type: EC 3.2.1.23

Research Articles on lacZ

1. Report the solution structure at approximately 3.2-A resolution by using single-particle cryo-electron microscopy (cryo-EM). Densities for most side chains, including those of residues in the active site, and a catalytic Mg(2+) ion can be discerned.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with lacZ active protein

Reference Product	PubMed Publications
crp active protein	>150 publications with lacZ and crp
lacI active protein	>123 publications with lacZ and lacI
lexA active protein	>119 publications with lacZ and lexA
lacY active protein	>44 publications with lacZ and lacY
lrp active protein	>37 publications with lacZ and lrp
soxR active protein	>34 publications with lacZ and soxR
galK active protein	>34 publications with lacZ and galK
ompA active protein	>23 publications with lacZ and ompA
degP active protein	>22 publications with lacZ and degP
lacA active protein	>21 publications with lacZ and lacA

Pathways associated with lacZ active protein

Products by Pathway	Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Other Glycan Degradation Pathway antibodies	Other Glycan Degradation Pathway Diagram
Other Glycan Degradation Pathway antibodies	Other Glycan Degradation Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Lactose Degradation III Pathway antibodies	Lactose Degradation III Pathway Diagram
Lactose Degradation III Pathway antibodies	Lactose Degradation III Pathway Diagram