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TNF ALPHA BOVINE Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS232226

Bovine Tumour Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response. MBS232226 has an ED50 in the range of 10-25 ng/ml,… More details

$1140 / 0.025 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TNF ALPHA, Recombinant Protein
Full Product Name
RECOMBINANT BOVINE TNF ALPHA
Form/Format
Purified Recombinant Protein- lyophilised
Purity/Purification
>95% by SDS PAGE analysis
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -70 degree C. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
Protein Molecular Weight: 17.4kD
3D Structure
ModBase 3D Structure for Q06599
Reconstitution
Reconstitute with 0.5 ml distilled water. Further dilutions should be made in a buffer containing carrier protein.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Preparation||Recombinant protein expressed in Pichia pastoris
Endotoxin Level
less than 0.7 EU/ug
Buffer Solution||Phosphate buffered saline
Target Species
Bovine
Applications Tested/Suitable for TNF ALPHA recombinant protein
ELISA (EIA), Functional Assays (FN)

Other Notes

Small volumes of TNF ALPHA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNF ALPHA purchase

MBS232226 is a recombinant protein targeting TNF ALPHA. Also known as RECOMBINANT BOVINE TNF ALPHA.

Additional Details

NCBI/Uniprot data below describe general gene information for TNF ALPHA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor<
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
TNFa; TNF-a; TNF-alpha
UniProt Protein Name
Tumor necrosis factor
UniProt Synonym Protein Names
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_BOVIN
NCBI Protein Information
tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2

UniProt Comments for TNF ALPHA

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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