HUMAN DEFB4B Active Protein – Cat# MBS232312

DEFENSIN BETA 2 (DEFB4B) HUMAN Active Protein

Name: DEFB4B Active Protein; DEFENSIN BETA 2; HUMAN; MyBioSource
Brand: MyBioSource
SKU: MBS232312
Price: 590 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS232312

Recombinant human defensin beta-2 (BD-2), is a 4.3 kDa peptide comprised of 41 amino acid residues. BD-2, a member of the beta defensin family, is secreted at epithelial surfaces of the skin and respiratory tract and by some leukocytes. This defensin is… More details

$590 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DEFENSIN BETA 2 (DEFB4B), Active Protein

Full Product Name

RECOMBINANT HUMAN DEFENSIN BETA 2

Product Gene Name

DEFB4B active protein

Sequence

GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P

Form/Format

Purified recombinant protein - lyophilised

Purity/Purification

>98% by SDS PAGE and HPLC analysis.

Concentration

Approximate Protein Concentration: 0.1mg/ml after reconstitution (lot specific)

Preparation and Storage

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

Molecular Weight

4.3kD (41 amino acid sequence/residues)

OMIM

602215

3D Structure

ModBase 3D Structure for O15263

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Reconstitution

Reconstitute with 0.2ml 10mM acetic acid. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation||Purified recombinant BD-2, expressed in E Coli

Activity

Determined by its ability to chemoattract immature dendritic cells using a concentration of 10.0-100.0 ng/ml.

Target Species

Human

Applications Tested/Suitable for DEFB4B active protein

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

Application Notes for DEFB4B active protein

ELISA: Recombinant human BD-2 may be used as a standard in ELISA applications with either a purified human BD-2 antibody or a biotinylated human BD-2 antibody.
Western Blot: Recombinant human BD-2 may be used as the positive control in Western Blotting applications with either a purified human BD-2 antibody or a biotinylated human BD-2 antibody.
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well
Functional Assays: Minimum Dilution: 10; Maximum Dilution: 100ng/ml
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

Other Notes

Small volumes of DEFB4B active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEFB4B purchase

MBS232312 is an active protein targeting DEFB4B. Also known as RECOMBINANT HUMAN DEFENSIN BETA 2.

Additional Details

NCBI/Uniprot data below describe general gene information for DEFB4B. It may not necessarily be applicable to this product.

NCBI GI #

327532771

NCBI GeneID

100289462

NCBI Accession #

NP_001192195.1

UniProt Primary Accession #

O15263

UniProt Secondary Accession #

Q52LC0

GenBank Nucleotide #

NM_001205266.1

NCBI Official Full Name

beta-defensin 4B

NCBI Official Synonym Full Names

defensin, beta 4B

NCBI Official Symbol

DEFB4B

NCBI Official Synonym Symbols

DEFB4P

UniProt Protein Name

Beta-defensin 4A

UniProt Synonym Protein Names

Beta-defensin 2; BD-2; hBD-2; Defensin, beta 2; Skin-antimicrobial peptide 1; SAP1

Protein Family

Beta-defensin

UniProt Gene Name

DEFB4A

UniProt Synonym Gene Names

DEFB102; DEFB2; DEFB4; BD-2; hBD-2; SAP1

UniProt Entry Name

DFB4A_HUMAN

NCBI Protein Information

beta-defensin 4B; beta defensin 2; beta defensin-2; beta-defensin 2; defensin, beta 4, pseudogene

NCBI Summary for DEFB4B

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Oct 2014]

UniProt Comments for DEFB4B

defensin, beta 2: Has antibacterial activity (Potential). Belongs to the beta-defensin family. LAP/TAP subfamily.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: Golgi lumen; extracellular region

Biological Process: G-protein coupled receptor protein signaling pathway; defense response to bacterium; innate immune response; immune response; chemotaxis

Research Articles on DEFB4B

1. The encoded peptide has antimicrobial activity against E.coli, S. aureus, and P. aeruginosa.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with DEFB4B active protein

Products by Pathway	Pathway Diagram
Beta Defensins Pathway antibodies	Beta Defensins Pathway Diagram
Defensins Pathway antibodies	Defensins Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram