MOUSE MIP-1 ALPHA Recombinant Protein – Cat# MBS232334

MIP-1 ALPHA MOUSE Recombinant Protein

Name: MIP-1 ALPHA Recombinant Protein; MOUSE; MyBioSource
Brand: MyBioSource
SKU: MBS232334
Price: 585 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS232334

Murine macrophage inflammatory protein-1 alpha (MIP-1 alpha), also known as CCL3, is a member of the chemokine family (C-C) and plays an important role in the inflammatory response of blood monocytes and tissue macrophages. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MIP-1 ALPHA, Recombinant Protein

Full Product Name

RECOMBINANT MOUSE MIP-1 ALPHA

Product Gene Name

MIP-1 ALPHA recombinant protein

Form/Format

Purified recombinant protein - lyophilised

Purity/Purification

>98% by SDS PAGE and HPLC analysis

Concentration

Approximate Protein Concentration: 0.1 mg/ml after reconstitution (lot specific)

Preparation and Storage

Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

Molecular Weight

Protein Molecular Weight: 7.8kD (69 amino acid sequence)

3D Structure

ModBase 3D Structure for P10855

Reconstitution

Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.1% bovine serum albumin is recommended.
Preparation||Recombinant protein prepared from E Coli

Target Species

Mouse

Applications Tested/Suitable for MIP-1 ALPHA recombinant protein

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

Application Notes for MIP-1 ALPHA recombinant protein

ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;
Functional Assays: Minimum Dilution: 10; Maximum Dilution: 100ng/ml;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

Other Notes

Small volumes of MIP-1 ALPHA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIP-1 ALPHA purchase

MBS232334 is a recombinant protein targeting MIP-1 ALPHA. Also known as RECOMBINANT MOUSE MIP-1 ALPHA.

Additional Details

NCBI/Uniprot data below describe general gene information for MIP-1 ALPHA. It may not necessarily be applicable to this product.

NCBI GI #

6755432

NCBI GeneID

20302

NCBI Accession #

NP_035467.1

UniProt Primary Accession #

P10855

UniProt Secondary Accession #

P14096

UniProt Related Accession #

P10855

GenBank Nucleotide #

NM_011337.2

NCBI Official Full Name

C-C motif chemokine 3

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 3

NCBI Official Symbol

Ccl3

NCBI Official Synonym Symbols

Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha

UniProt Protein Name

C-C motif chemokine 3

UniProt Synonym Protein Names

Heparin-binding chemotaxis protein; L2G25B; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; SIS-alpha; Small-inducible cytokine A3; TY-5

Protein Family

C-C motif chemokine

UniProt Gene Name

Ccl3

UniProt Synonym Gene Names

Mip1a; Scya3; MIP-1-alpha

UniProt Entry Name

CCL3_MOUSE

NCBI Protein Information

C-C motif chemokine 3; L2G25B; MIP-1 alpha; MIP-1-alpha; MIP1 (a); SIS-alpha; TY-5; heparin-binding chemotaxis protein; macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; small inducible cytokine A3; small-inducible cytokine A3

UniProt Comments for MIP-1 ALPHA

CCL3L1: Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1- infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine; Secreted

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: protein binding; chemokine activity; cytokine activity

Biological Process: neutrophil chemotaxis; positive regulation of osteoclast differentiation; leukocyte chemotaxis; positive regulation of interleukin-1 beta secretion; chemotaxis; positive regulation of tumor necrosis factor production; macrophage chemotaxis; regulation of cell shape; elevation of cytosolic calcium ion concentration; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; astrocyte cell migration; positive regulation of inflammatory response

Research Articles on MIP-1 ALPHA

1. Negative modulation of PGE2 signaling reduced infection-induced anti-inflammatory cytokine polarization and enhanced inflammatory chemokines, CCL3 and CCL5.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with MIP-1 ALPHA recombinant protein

Products by Pathway	Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram

Diseases associated with MIP-1 ALPHA recombinant protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>892 publications with MIP-1 ALPHA and Inflammation
Necrosis Antibodies	>506 publications with MIP-1 ALPHA and Necrosis
Disease Models, Animal Antibodies	>314 publications with MIP-1 ALPHA and Disease Models, Animal
Lung Diseases Antibodies	>238 publications with MIP-1 ALPHA and Lung Diseases
HIV Infections Antibodies	>188 publications with MIP-1 ALPHA and HIV Infections
Cardiovascular Diseases Antibodies	>172 publications with MIP-1 ALPHA and Cardiovascular Diseases
Hypersensitivity Antibodies	>105 publications with MIP-1 ALPHA and Hypersensitivity
Liver Diseases Antibodies	>95 publications with MIP-1 ALPHA and Liver Diseases
Fibrosis Antibodies	>79 publications with MIP-1 ALPHA and Fibrosis
Pneumonia Antibodies	>72 publications with MIP-1 ALPHA and Pneumonia

Organs/Tissues associated with MIP-1 ALPHA recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1552 publications with MIP-1 ALPHA and Blood
Bone Antibodies	>291 publications with MIP-1 ALPHA and Bone
Brain Antibodies	>257 publications with MIP-1 ALPHA and Brain
Bone Marrow Antibodies	>208 publications with MIP-1 ALPHA and Bone Marrow
Spleen Antibodies	>111 publications with MIP-1 ALPHA and Spleen
Skin Antibodies	>101 publications with MIP-1 ALPHA and Skin
Lymph Node Antibodies	>94 publications with MIP-1 ALPHA and Lymph Node
Joint Antibodies	>74 publications with MIP-1 ALPHA and Joint
Embryonic Tissue Antibodies	>30 publications with MIP-1 ALPHA and Embryonic Tissue
Thymus Antibodies	>21 publications with MIP-1 ALPHA and Thymus