Il-6 (IL6) Active Protein

Name: IL6 Active Protein; Il-6; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS232383
Price: 585 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS232383

Recombinant rat IL-6 is a 21.7 kDa peptide containing 188 residues corresponding to full-length mature rat interleukin-6. IL-6 is a pleiotropic cytokine involved in the regulation of immune and inflammatory responses. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

IL-6 (Il6), Active Protein

Full Product Name

RECOMBINANT RAT INTERLEUKIN-6

Product Gene Name

Il6 active protein

Form/Format

Purified recombinant protein expressed in E Coli - lyophilised.

Purity/Purification

>98% by SDS PAGE and HPLC analysis.

Concentration

Approximate Protein Concentration: 0.1 mg/ml after reconstitution (lot specific)

Preparation and Storage

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life: 3 months from date of reconstitution.

Molecular Weight

Protein Molecular Weight: 21.7kD (188 Amino acids)

3D Structure

ModBase 3D Structure for P20607

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Reconstitution

Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.

Buffer Solution

TRIS buffered saline
0.1% SDS

Activity

ED50 of < 0.01ng/ml when assayed by proliferation of IL-6 dependent murine 7TD1 cells. This corresponds to a specific activity of 1 x 108 units/mg.

Target Species

Rat

Applications Tested/Suitable for Il6 active protein

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

Application Notes for Il6 active protein

ELISA: Recombinant rat IL-6 may be used as a standard for ELISA applications with either anti Rat IL-6: purified or anti Rat IL-6: biotin conjugate.
Western Blot: Recombinant Rat IL-6 may be used as the positive control for western blotting applications with either anti Rat IL-6: purified or anti Rat IL-6: biotin conjugate.
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well
Functional Assays: Minimum Dilution: 0.01; Maximum Dilution: 10ng/ml
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

Other Notes

Small volumes of Il6 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Il6 purchase

MBS232383 is an active protein targeting Il6. Also known as RECOMBINANT RAT INTERLEUKIN-6.

Additional Details

NCBI/Uniprot data below describe general gene information for Il6. It may not necessarily be applicable to this product.

NCBI GI #

7549769

NCBI GeneID

24498

NCBI Accession #

NP_036721.1

UniProt Primary Accession #

P20607

UniProt Related Accession #

P20607

GenBank Nucleotide #

NM_012589.2

NCBI Official Full Name

interleukin-6

NCBI Official Synonym Full Names

interleukin 6

NCBI Official Symbol

Il6

NCBI Official Synonym Symbols

ILg6; Ifnb2

UniProt Protein Name

Interleukin-6

Protein Family

Interleukin

UniProt Gene Name

Il6

UniProt Synonym Gene Names

Il-6; IL-6

UniProt Entry Name

IL6_RAT

NCBI Protein Information

interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)

NCBI Summary for Il6

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

UniProt Comments for Il6

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().

Research Articles on Il6

1. The objective of this study is to investigate the possible role of inflammatory mediators such as IL-6, IL-1beta, and TNF-alpha in Kashin-Beck disease (KBD) children and rats fed with T-2 toxin under a selenium-deficient nutrition.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Il6 active protein

Reference Product	PubMed Publications
Tnf active protein	>1470 publications with Il6 and Tnf
Crp active protein	>483 publications with Il6 and Crp
Stat3 active protein	>389 publications with Il6 and Stat3
Icam1 active protein	>153 publications with Il6 and Icam1
Rela active protein	>63 publications with Il6 and Rela
Il6st active protein	>46 publications with Il6 and Il6st
Fos active protein	>45 publications with Il6 and Fos
Il12b active protein	>38 publications with Il6 and Il12b
Nfkb1 active protein	>31 publications with Il6 and Nfkb1
Il6ra active protein	>1 publications with Il6 and Il6ra

Pathways associated with Il6 active protein

Products by Pathway	Pathway Diagram
ARMS-mediated Activation Pathway antibodies	ARMS-mediated Activation Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Cellular Senescence Pathway antibodies	Cellular Senescence Pathway Diagram

Diseases associated with Il6 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>2394 publications with Il6 and Inflammation
Necrosis Antibodies	>1731 publications with Il6 and Necrosis
Autoimmune Diseases Antibodies	>452 publications with Il6 and Autoimmune Diseases
Obesity Antibodies	>298 publications with Il6 and Obesity
Skin Diseases Antibodies	>296 publications with Il6 and Skin Diseases
Arthritis, Rheumatoid Antibodies	>268 publications with Il6 and Arthritis, Rheumatoid
Atherosclerosis Antibodies	>244 publications with Il6 and Atherosclerosis
Myocardial Ischemia Antibodies	>230 publications with Il6 and Myocardial Ischemia
Ischemia Antibodies	>117 publications with Il6 and Ischemia
Diabetes Mellitus, Type 2 Antibodies	>111 publications with Il6 and Diabetes Mellitus, Type 2

Organs/Tissues associated with Il6 active protein

Organ/Tissue Name	Pubmed Publications
Uncharacterized Tissue Antibodies	>1 publications with Il6 and Uncharacterized Tissue