HUMAN DEFB103A Active Protein – Cat# MBS232468

DEFENSIN BETA 3 (DEFB103A) HUMAN Active Protein

Name: DEFB103A Active Protein; DEFENSIN BETA 3; HUMAN; MyBioSource
Brand: MyBioSource
SKU: MBS232468
Price: 585 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS232468

Defensin beta 3 (BD-3) is a cationic non-haemolytic antimicrobial peptide and member of the beta-defensin family, highly expressed by skin and tonsils, which plays an important role in the innate immune response to potentially pathogenic microbes, including… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

DEFENSIN BETA 3 (DEFB103A), Active Protein

Full Product Name

RECOMBINANT HUMAN DEFENSIN BETA 3

Product Gene Name

DEFB103A active protein

Form/Format

Purified recombinant protein - lyophilised

Purity/Purification

>98% by SDS PAGE and HPLC analysis

Concentration

Approximate Protein Concentration: 1.0 mg/ml after reconstitution (lot specific)

Preparation and Storage

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

Molecular Weight

5.1kD (45 amino acid residues)

OMIM

606611

3D Structure

ModBase 3D Structure for P81534

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Reconstitution

Reconstitute with 20ul 10mM acetic acid. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. N.B. For long term storage, further dilutions may be made in buffer containing 0.1% bovine serum albumin (BSA).
Preparation||Purified recombinant defensin beta 3 expressed in E Coli

Activity

Exhibits chemotactic activity on monocytes.

Target Species

Human

Applications Tested/Suitable for DEFB103A active protein

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

Application Notes for DEFB103A active protein

ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

Other Notes

Small volumes of DEFB103A active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEFB103A purchase

MBS232468 is an active protein targeting DEFB103A. Also known as RECOMBINANT HUMAN DEFENSIN BETA 3.

Additional Details

NCBI/Uniprot data below describe general gene information for DEFB103A. It may not necessarily be applicable to this product.

NCBI GI #

125656159

NCBI GeneID

414325

NCBI Accession #

NP_001075020.1

UniProt Primary Accession #

P81534

UniProt Secondary Accession #

Q8NFG6; Q9NPF6

UniProt Related Accession #

P81534

GenBank Nucleotide #

NM_001081551.3

NCBI Official Full Name

beta-defensin 103

NCBI Official Synonym Full Names

defensin, beta 103A

NCBI Official Symbol

DEFB103A

NCBI Official Synonym Symbols

BD-3; HBD3; HBP3; DEFB3; HBP-3; hBD-3; DEFB-3; DEFB103

UniProt Protein Name

Beta-defensin 103

UniProt Synonym Protein Names

Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein

Protein Family

Beta-defensin

UniProt Gene Name

DEFB103A

UniProt Synonym Gene Names

BD3; DEFB103; DEFB3; BD-3; DEFB-3; HBD3; hBD-3

UniProt Entry Name

D103A_HUMAN

NCBI Protein Information

beta-defensin 103; beta defensin 3; beta defensin-3; beta-defensin 3; defensin, beta 103; defensin, beta 3; defensin-like protein

NCBI Summary for DEFB103A

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, an antibiotic peptide which is induced by bacteria and interferon gamma, and which displays antimicrobial activity against S. aureus, S. pyogenes, P. aeruginosa, E. coli, and C. albicans. [provided by RefSeq, Oct 2014]

UniProt Comments for DEFB103A

defensin, beta 3: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. Belongs to the beta-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: Golgi lumen; extracellular region

Biological Process: defense response to bacterium; innate immune response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria

Research Articles on DEFB103A

1. The encoded peptide displays antimicrobial activity against S. aureus and E. faecium.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DEFB103A active protein

Reference Product	PubMed Publications
CAMP active protein	>2 publications with DEFB103A and CAMP
DEFB104A active protein	>2 publications with DEFB103A and DEFB104A

Pathways associated with DEFB103A active protein

Products by Pathway	Pathway Diagram
Beta Defensins Pathway antibodies	Beta Defensins Pathway Diagram
Defensins Pathway antibodies	Defensins Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram

Diseases associated with DEFB103A active protein

Disease Name	Pubmed Publications
Metabolic Diseases Antibodies	>1 publications with DEFB103A and Metabolic Diseases