HUMAN CD54 Active Protein – Cat# MBS232503

CD54 HUMAN Active Protein

Name: CD54 Active Protein; HUMAN; MyBioSource
Brand: MyBioSource
SKU: MBS232503
Price: 585 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS232503

CD54, otherwise known as ICAM-1, is a widely expressed type I integral membrane glycoprotein and member of the immunoglobulin superfamily, which acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, and is the major receptor for… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CD54, Active Protein

Full Product Name

RECOMBINANT HUMAN CD54

Product Gene Name

CD54 active protein

Form/Format

Purified recombinant protein - lyophilised

Purity/Purification

>98% by SDS PAGE and HPLC analysis

Concentration

Approximate Protein Concentration: 0.1mg/ml after reconstitution (lot specific)

Preparation and Storage

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

Molecular Weight

49.5kD (453 amino acid residues)

OMIM

147840

3D Structure

ModBase 3D Structure for P05362

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Reconstitution

Reconstitute with 0.5ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Preparation||Purified recombinant CD54 expressed in CHO cells

Buffer Solution

10mM Sodium Phosphate

Activity

CD54 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at a CD54 concentration of 25ng/ml.

Target Species

Human

Applications Tested/Suitable for CD54 active protein

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

Application Notes for CD54 active protein

ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

Other Notes

Small volumes of CD54 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD54 purchase

MBS232503 is an active protein targeting CD54. Also known as RECOMBINANT HUMAN CD54.

Additional Details

NCBI/Uniprot data below describe general gene information for CD54. It may not necessarily be applicable to this product.

NCBI GI #

167466198

NCBI GeneID

3383

NCBI Accession #

NP_000192.2

UniProt Primary Accession #

P05362

UniProt Secondary Accession #

Q5NKV7; Q96B50; B2R6M3

UniProt Related Accession #

P05362

GenBank Nucleotide #

NM_000201.2

NCBI Official Full Name

intercellular adhesion molecule 1

NCBI Official Synonym Full Names

intercellular adhesion molecule 1

NCBI Official Symbol

ICAM1

NCBI Official Synonym Symbols

BB2; CD54; P3.58

UniProt Protein Name

Intercellular adhesion molecule 1

UniProt Synonym Protein Names

Major group rhinovirus receptor; CD_antigen: CD54

Protein Family

Intercellular adhesion molecule

UniProt Gene Name

ICAM1

UniProt Synonym Gene Names

ICAM-1

UniProt Entry Name

ICAM1_HUMAN

NCBI Protein Information

intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor

NCBI Summary for CD54

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

UniProt Comments for CD54

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane

Molecular Function: viral receptor activity; integrin binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: entry of virus into host cell; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; cellular response to nutrient levels; leukocyte adhesion; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; response to drug; regulation of cell adhesion; virion attachment, binding of host cell surface receptor; regulation of immune response; negative regulation of calcium ion transport; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; positive regulation of actin filament polymerization; response to copper ion; positive regulation of vasoconstriction; adhesion to host; cell adhesion mediated by integrin; response to ionizing radiation; leukocyte migration

Disease: Malaria, Susceptibility To

Research Articles on CD54

1. ICAM1 is likely to be involved in the development of childhood asthma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD54 active protein

Reference Product	PubMed Publications
ITGB2 active protein	>880 publications with CD54 and ITGB2
ITGAL active protein	>474 publications with CD54 and ITGAL
ITGAM active protein	>440 publications with CD54 and ITGAM
CD44 active protein	>371 publications with CD54 and CD44
IL6 active protein	>153 publications with CD54 and IL6
ITGAX active protein	>139 publications with CD54 and ITGAX
SELE active protein	>87 publications with CD54 and SELE
HLA-B active protein	>29 publications with CD54 and HLA-B
DIF active protein	>3 publications with CD54 and DIF

Pathways associated with CD54 active protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with CD54 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>5639 publications with CD54 and Inflammation
Necrosis Antibodies	>4797 publications with CD54 and Necrosis
Cardiovascular Diseases Antibodies	>3657 publications with CD54 and Cardiovascular Diseases
Atherosclerosis Antibodies	>1470 publications with CD54 and Atherosclerosis
Reperfusion Injury Antibodies	>884 publications with CD54 and Reperfusion Injury
Myocardial Ischemia Antibodies	>797 publications with CD54 and Myocardial Ischemia
Kidney Diseases Antibodies	>760 publications with CD54 and Kidney Diseases
Hypertension Antibodies	>527 publications with CD54 and Hypertension
Asthma Antibodies	>502 publications with CD54 and Asthma
Stroke Antibodies	>388 publications with CD54 and Stroke

Organs/Tissues associated with CD54 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>11797 publications with CD54 and Blood
Vascular Antibodies	>8826 publications with CD54 and Vascular
Lung Antibodies	>1776 publications with CD54 and Lung
Heart Antibodies	>1288 publications with CD54 and Heart
Skin Antibodies	>1198 publications with CD54 and Skin
Liver Antibodies	>1180 publications with CD54 and Liver
Kidney Antibodies	>1177 publications with CD54 and Kidney
Brain Antibodies	>1177 publications with CD54 and Brain
Muscle Antibodies	>1049 publications with CD54 and Muscle
Embryonic Tissue Antibodies	>774 publications with CD54 and Embryonic Tissue