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IgG Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS235192

Mouse IgG (all subclasses). Some cross reactivity with IgM will be expected. Cross reactivity is expected with rat IgG. Cross reactivity with human serum has been minimised by solid phase adsorbtion. More details

$1020 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG, Polyclonal Antibody
Full Product Name
RABBIT F(ab')2 ANTI MOUSE IgG:HRP (Human Adsorbed)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
HRP
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Specificity
Mouse IgG (all subclasses). Some cross reactivity with IgM will be expected. Cross reactivity is expected with rat IgG. Cross reactivity with human serum has been minimised by solid phase adsorbtion.
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
35,705 Da
Perservative Stabilisers
0.01% Thiomersal
HRP Stabiliser
Preparation||Purified IgG framents were prepared by affinity chromatography of serum. F(ab)2 fragments were prepared by pepsin digestion, followed by gel filtration to remove any intact IgG or Fc fragments.
Immunogen
Purified whole mouse immunoglobulin G
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-IgG antibody
Immunohistology Frozen, ELISA (EIA), Immunohistology Paraffin, Immunohistology Resin (RE), Western Blot (WB)
Application Notes for anti-IgG antibody
Immunohistology - Frozen: Maximum Dilution: 1/50;
ELISA: Minimum Dilution: 1/500; Maximum Dilution: 1/1000;
Immunohistology - Paraffin: Maximum Dilution: 1/50;
Immunohistology - Resin: Maximum Dilution: 1/50;
Western Blotting: Minimum Dilution: 1/2000; Maximum Dilution: 1/5000

Testing Data #1 of anti-IgG antibody

Human insulin like growth factor I detected with Mouse anti Human Insulin Like Growth Factor I followed by Rabbit F(ab')2 anti Mouse IgG:HRP (MBS235192)
Rabbit Anti-IgG Polyclonal Antibody – Cat# MBS235192

Testing Data #2 of anti-IgG antibody

Detection of Mouse anti Human alkaline phosphatase (MBS211427) by Rabbit (Fab')2 anti Mouse IgG:HRP (MBS235192) in a Western blot analysis of a bacterial alkaline phosphatase tagged HuCAL antibody (HCA106)
Rabbit Anti-IgG Polyclonal Antibody, Testing Data #2 – Cat# MBS235192

Testing Data #3 of anti-IgG antibody

Detection of Mouse anti Strep-tag by Rabbit (Fab')2 anti Mouse IgG:HRP (MBS235192) in a Western Blot analysis of Human anti Human Ku70 (HCA127) on a HeLa cell lysate
Rabbit Anti-IgG Polyclonal Antibody, Testing Data #3 – Cat# MBS235192

Testing Data #4 of anti-IgG antibody

Detection of Mouse anti Human synaptobrevin (MBS212092) by Rabbit (Fab')2 anti Mouse IgG:HRP (MBS235192) in a Western blot analysis of synaptobrevin expression in a human brain lysate
Rabbit Anti-IgG Polyclonal Antibody, Testing Data #4 – Cat# MBS235192

Testing Data #5 of anti-IgG antibody

Detection of Mouse anti Human luteinizing hormone clones 157.10H10 and 156.10B7 at dilutions indicated on the x-axis with coating antigen at 5ugml and Rabbit F(ab')2 anti Mouse IgG:HRP (MBS235192) as the detection reagent
Rabbit Anti-IgG Polyclonal Antibody, Testing Data #5 – Cat# MBS235192

Other Notes

Small volumes of anti-IgG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IgG purchase

MBS235192 is an antibody targeting IgG. Also known as RABBIT F(ab')2 ANTI MOUSE IgG:HRP (Human Adsorbed). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IgG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
Ig gamma-1 chain C region, membrane-bound form, partial
UniProt Protein Name
Ig gamma-1 chain C region, membrane-bound form
Protein Family
UniProt Gene Name
Ighg1
UniProt Synonym Gene Names
Igh-4
UniProt Entry Name
IGH1M_MOUSE

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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