ANTIGEN UNMASKING FLUID Accessory Reagent – UNIVERSAL, Concentrate

Concentrate

Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Mix 10ml with 990ml distilled water
Intended Use||MyBioSource's Antigen unmasking fluid is a heat induced, epitope retrieval system. The 'universal' formulation presented at pH8.0 is suitable for use with most antibodies and gives excellent preservation of tissue morphology. MyBioSource also offers two other versions, an acidic and basic form, which may be of value for use with antibodies that do not work well with this reagent. These buffers may dramatically increase staining intensity but may also have an effect upon tissue morphology.
Instructions For Use||1. Deparaffinise sections and re-hydrate

2. Pre-heat retrieval buffer to 92 degree C *.

3. Place slides in buffer and incubate 10 minutes at 92 degree C *.

4. Allow to cool for 5-10 minutes.

5. Rinse in distilled water or PBS, proceed with staining.

* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90 degree C-100 degree C) and time (5-30 minutes) should be determined by each investigator.

100x