RED CELL LYSING BUFFER Accessory Reagent – 10x, Reagent

Reagent

The 10X concentrated ERYTHROLYSE should be stored at room temperature and is stable for 1 year. Do not use if any discolouration or precipitation occurs in the ERYTHROLYSE solution. The information regarding the products is believed to be accurate, but the information and products have no warranty or guarantee other than those specified, since the ultimate conditions of use and the
variability of the materials are beyond our control. We cannot be responsible for and we do not intend these products or the accompanying information to be used for patent infringement or other such violations
Shelf Life: 1 year from the date of despatch.

MyBioSource ERYTHROLYSE is supplied as a 10X concentration solution which must be diluted 1/10 with distilled water before use.

MyBioSource ERYTHROLYSE is designed for use in whole blood immunofluorescent staining procedures and is suitable for use with human, rat and mouse blood.

Following staining according to standard procedures (worksheets available on request), 2ml of diluted ERYTHROLYSE is added to 100ul of whole blood, mixed well and incubated for 10 minutes at room temperature. The tubes should then be centrifuged (400g, 5 minutes), the supernatant decanted and cells washed once in 2ml PBS/1% BSA before analysis by flow cytometry.