TMB Accessory Reagent

Ready To Use
Ready to use TMB solution - liquid

Store at 4 degree C. DO NOT FREEZE. This product is photosensitive and should be protected from light. Avoid exposure to heat and contamination with metal ions or peroxidase. Store in bottles made of High Density Polyethylene (HDPE).
Shelf Life: 24 months from date of despatch.

This item is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP).

This item contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. TMB SIGNAL+ has been optimised to enable increased sensitivity whilst maintaining increased stability. This item provides minimal background and rapid development.

This item produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the
reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
Instructions For Use||1. Pour the desired amount of this item into a sealable container and allow it to reach room temperature in the dark. Note that 0.1ml will be required per well.

2. Add 0.1ml of This item per microtiter well.

3. Allow development of This item. Development time is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation. i.e. wrapped in tinfoil.

4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.

5. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of This item is not recommended).