QUANTUM FITC-5 MESF Accessory Reagent

Flow Cytometry Calibration Reagent

Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life: 12 months from date of despatch.

1 x 1ml bottle of unlabeled microbeads 5 x 1ml bottles of labeled microbeads
Perservative Stabilisers||0.05% Sodium Azide (NaN3)
0.01% Tween 80 (unlabeled only)
Intended Use||This item is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit have been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:1) quantitation of fluorescence intensity of samples in terms of MESF;2) determination of instrument fluorescence detection threshold;3) determination of instrument linearity;4) data comparison over time and between multiple instruments.

Phosphate buffered saline