Mitochondrial Permeability Transition:mitopttm Jc-1 KIT (MITOCHONDRIAL) Assay Kit

Description: The MitoPTTM JC-1 kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality.
Test Principle: An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzamidazolocarbocyanin iodide) that has been incorporated into the MitoPTTM JC-1 kit.

The MitoPTTM JC-1 reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This allows an easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Small volumes of MITOCHONDRIAL PERMEABILITY TRANSITION:MitoPT^TM JC-1 KIT assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS239197 is an assay kit targeting MITOCHONDRIAL PERMEABILITY TRANSITION:MitoPT^TM JC-1 KIT. Also known as MITOCHONDRIAL PERMEABILITY TRANSITION KIT.