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GPC1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2400985

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates inSchwann cell myelination (By similarity). May act as a catalyst inincreasing the rate of conversion of prion protein… More details

$715 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GPC1/Glypican, Polyclonal Antibody
Full Product Name
Anti-GPC1/Glypican Antibody (Internal)
Product Gene Name
Product Synonym Names
Mouse Monoclonal (IgG1, k) to Human GPC1/Glypican; GPC1; Glypican 1; Glypican; Glypican-1; Glypican proteoglycan 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Predicted: Mouse, Rat
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Purity/Purification
Protein A purified
Specificity
This antibody is specific for human glypican-1 protein. A BLAST analysis was used to suggest partial cross-reactivity with glypican from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 - 88% homology with the immunizing sequence. Cross-reactivity with glypican from other sources has not been determined.
Preparation and Storage
Short Term: Store at 4 degree C
Long Term: Aliquot and store at -20 degree C
Avoid freeze thaw cycles.
Molecular Weight
31,931 Da
OMIM
600395
3D Structure
ModBase 3D Structure for P35052
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
Conjugation
Unconjugated
Epitope
Internal
Applications Tested/Suitable for anti-GPC1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-GPC1 antibody
ELISA: 1:10000-1:50000
IHC-P: 10 ug/ml
WB: 1:500-1:2000

Immunohistochemistry (IHC) of anti-GPC1 antibody

Anti-GPC1/Glypican antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-GPC1 Polyclonal Antibody – Cat# MBS2400985

Western Blot (WB) of anti-GPC1 antibody

Anti-Glypican-1 Antibody - Western Blot. Western blot of protein A purified anti-Glypican-1 antibody shows detection of exogenous glypican-1 in 50 ug 293T whole cell lysate (lane 2). Minor bands may represent post translational modifications of glypican-1. The blot was incubated overnight with primary antibody at a 1:1000 dilution followed by washes and exposure to the secondary antibody HRP Gt-a-Rabbit IgG diluted 1:5000 in 5% BLOTTO at 4C. Lane 1: untransfected 293T cell lysate. Personal Communication, Caterina Bianco, CCR-NCI, Bethesda, MD.
Rabbit Anti-GPC1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2400985

Other Notes

Small volumes of anti-GPC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPC1 purchase

MBS2400985 is an antibody targeting GPC1. Also known as Anti-GPC1/Glypican Antibody (Internal). Reactive in Human
Predicted: Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GPC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glypican-1
NCBI Official Synonym Full Names
glypican 1
NCBI Official Symbol
GPC1
NCBI Official Synonym Symbols
glypican
UniProt Protein Name
Glypican-1
Protein Family
UniProt Gene Name
GPC1
NCBI Protein Information
glypican-1

NCBI Summary for GPC1

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]
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UniProt Comments for GPC1

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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