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LGALS3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2401012

LGALS3/Galectin 3 is a member of the galectin family of carbohydratebinding proteins. Members of this protein family have an affinity forbeta-galactosides. the encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single… More details

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Product Overview

Online Datasheet in PDF
Product Name
LGALS3/Galectin 3, Monoclonal Antibody
Full Product Name
Anti-LGALS3/Galectin 3 Antibody (clone 52C1)
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone A3A12] (IgG1) to Human LGALS3/Galectin 3; LGALS3; 35 kd lectin; 35 kDa lectin; CBP35; GAL3; Galactoside-binding protein; GALIG; IgE-binding protein; Laminin-binding protein; Gal-3; GALBP; MAC-2; Mac-2 antigen; L31; MAC2; CBP 35; Galactose-specific lectin 3; Galectin-3; L-31; Lectin L-29
Clonality
Monoclonal
Isotype
IgG1
Clone Number
52C1
Host
Mouse
Species Reactivity
Human
Predicted: Mouse, Rat
Form/Format
PBS, 10 mM sodium azide.
Purity/Purification
Affinity Purified
Specificity
Clone 5C21 is known to react with Galectin-3 from human, mouse and rat. Since Galectin-3 is highly conserved, it is likely that the antibody is effective on other species also.
Preparation and Storage
Short term storage: 4 degree C
Long term storage: aliquot and store at -20 degree C, avoid freeze thaw cycles.
Store undiluted.
Molecular Weight
26,152 Da
OMIM
153619
3D Structure
ModBase 3D Structure for P17931
Immunogen
Full length recombinant Galectin-3 expressed in and purified from E. coli.
Conjugation
Unconjugated
Applications Tested/Suitable for anti-LGALS3 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-LGALS3 antibody
ICC: 1:1000
IF: 1:1000
IHC-P: 1:50
WB: 1:10000

Immunohistochemistry (IHC) of anti-LGALS3 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-LGALS3 Monoclonal Antibody – Cat# MBS2401012

Immunocytochemistry (ICC) of anti-LGALS3 antibody

Rat brain neural cultures stained with (green) and DNA (blue). Staining can be seen in several types of glia and lymphocytic cells, including these cells which have the morphology of microglia. Surrounding cells reveal no Galectin-3 staining.
Mouse Anti-LGALS3 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS2401012

Western Blot (WB) of anti-LGALS3 antibody

Blots of crude HeLa cell extract stained with a panel of monoclonal antibodies to Galectin-3. Lane 12 was probed with revealing a band at the expected molecular weight of 30kDa.
Mouse Anti-LGALS3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS2401012

Other Notes

Small volumes of anti-LGALS3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LGALS3 purchase

MBS2401012 is an antibody targeting LGALS3. Also known as Anti-LGALS3/Galectin 3 Antibody (clone 52C1). Reactive in Human
Predicted: Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LGALS3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
galectin-3 isoform 1
NCBI Official Synonym Full Names
galectin 3
NCBI Official Symbol
LGALS3
NCBI Official Synonym Symbols
L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
UniProt Protein Name
Galectin-3
UniProt Synonym Protein Names
35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen
UniProt Gene Name
LGALS3
UniProt Synonym Gene Names
MAC2; Gal-3; CBP 35; GALBP
NCBI Protein Information
galectin-3

NCBI Summary for LGALS3

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
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UniProt Comments for LGALS3

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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