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MPS1/TTK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2401040

MPS1/TTK is a dual specificity protein kinase with the ability tophosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MPS1/TTK, Monoclonal Antibody
Full Product Name
Anti-MPS1/TTK Antibody (clone 3G7)
Product Gene Name
Product Synonym Names
Mouse Monoclonal (IgG1) to Human MPS1/TTK; TTK; Cancer/testis antigen 96; ESK; Esk1; MPS1; MPS1L1; Protein kinase esk; PYT; TTK protein kinase; CT96; Monopolar spindle 1-like 1
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
3G7
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4.
Purity/Purification
Purified from ascites by Protein A
Specificity
Human MPS1/TTK
Preparation and Storage
Store at -20 degree C or lower.
Aliquot to avoid repeated freezing and thawing.
Molecular Weight
96,944 Da
OMIM
604092
3D Structure
ModBase 3D Structure for P33981
Immunogen
TTK (AAH00633, aa1-176 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugation
Unconjugated

Product Categories/Family for anti-MPS1/TTK antibody

Applications Tested/Suitable for anti-MPS1/TTK antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-MPS1/TTK antibody
IHC-P: 10 ug/ml
WB: 1:500-1:1000

Immunohistochemistry (IHC) of anti-MPS1/TTK antibody

Anti-MPS1/TTK antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-MPS1/TTK Monoclonal Antibody – Cat# MBS2401040

Western Blot (WB) of anti-MPS1/TTK antibody

TTK monoclonal antibody (M01), clone 3G7. Western Blot analysis of TTK expression in HeLa.
Mouse Anti-MPS1/TTK Monoclonal Antibody, Western Blot (WB) – Cat# MBS2401040

Western Blot (WB) of anti-MPS1/TTK antibody

Western Blot analysis of TTK expression in transfected 293T cell line by TTK monoclonal antibody (M01), clone 3G7.Lane 1: TTK transfected lysate(97.1 KDa).Lane 2: Non-transfected lysate.
Mouse Anti-MPS1/TTK Monoclonal Antibody, Western Blot (WB) – Cat# MBS2401040

Western Blot (WB) of anti-MPS1/TTK antibody

TTK monoclonal antibody (M01), clone 3G7 Western Blot analysis of TTK expression in K-562.
Mouse Anti-MPS1/TTK Monoclonal Antibody, Western Blot (WB) – Cat# MBS2401040

ELISA (EIA) of anti-MPS1/TTK antibody

Detection limit for recombinant GST tagged TTK is approximately 0.3 ng/ml as a capture antibody.
Mouse Anti-MPS1/TTK Monoclonal Antibody, ELISA (EIA) – Cat# MBS2401040

Other Notes

Small volumes of anti-MPS1/TTK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MPS1/TTK purchase

MBS2401040 is an antibody targeting MPS1/TTK. Also known as Anti-MPS1/TTK Antibody (clone 3G7). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MPS1/TTK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dual specificity protein kinase TTK isoform 1
NCBI Official Synonym Full Names
TTK protein kinase
NCBI Official Symbol
TTK
NCBI Official Synonym Symbols
ESK; PYT; CT96; MPH1; MPS1; MPS1L1
UniProt Protein Name
Dual specificity protein kinase TTK
UniProt Synonym Protein Names
Phosphotyrosine picked threonine-protein kinase; PYT
UniProt Gene Name
TTK
UniProt Synonym Gene Names
MPS1; MPS1L1; PYT
NCBI Protein Information
dual specificity protein kinase TTK

NCBI Summary for MPS1/TTK

This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
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UniProt Comments for MPS1/TTK

Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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