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GLI2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240221

Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog… More details

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Product Overview

Online Datasheet in PDF
Product Name
GLI2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Mouse GLI2
Product Gene Name
Product Synonym Names
Anti-GLI2 Antibody (Internal) IHC-plus; GLI2; GLI-Kruppel family member GLI2; HPE9; Gli-2; Oncogene GLI2; Tax helper protein 2; THP; Zinc finger protein GLI2; Tax helper protein; THP2; GLI family zinc finger 2; Tax helper protein 1; THP1; Mouse GLI2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Amino acids from an internal region of Mouse Gli-2.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Molecular Weight
86,482 Da
OMIM
phenotype 615849
3D Structure
ModBase 3D Structure for P10070
Target Species
Mouse
Immunogen Description
Synthetic peptide from mouse GLI2.
Immunogen Type
Synthetic peptide
Immunogen
GLI2 antibody was raised against synthetic peptide from mouse GLI2.
Antigen Modification
Internal
Applications Tested/Suitable for anti-GLI2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-GLI2 antibody
ELISA (1:15000 - 1:60000), IHC-P (5 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...

Immunohistochemistry (IHC) of anti-GLI2 antibody

Anti-GLI2 antibody IHC of mouse, testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-GLI2 Polyclonal Antibody – Cat# MBS240221

Immunohistochemistry (IHC) of anti-GLI2 antibody

Anti-GLI2 antibody IHC of mouse, brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-GLI2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS240221

Western Blot (WB) of anti-GLI2 antibody

Western analysis of mouse brain whole lysate.
Rabbit Anti-GLI2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS240221

Other Notes

Small volumes of anti-GLI2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GLI2 purchase

MBS240221 is an antibody targeting GLI2. Also known as Rabbit Polyclonal to Mouse GLI2. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GLI2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
zinc finger protein GLI2
NCBI Official Synonym Full Names
GLI family zinc finger 2
NCBI Official Symbol
GLI2
NCBI Official Synonym Symbols
CJS; HPE9; PHS2; THP1; THP2
UniProt Protein Name
Zinc finger protein GLI2
UniProt Synonym Protein Names
GLI family zinc finger protein 2; Tax helper protein
Protein Family
UniProt Gene Name
GLI2
UniProt Entry Name
GLI2_HUMAN
NCBI Protein Information
zinc finger protein GLI2; oncogene GLI2; tax helper protein 1; tax helper protein 2; GLI-Kruppel family member GLI2; GLI family zinc finger protein 2; tax-responsive element-2 holding protein; glioma-associated oncogene family zinc finger 2; tax-responsive element-25-bp sequence binding protein

NCBI Summary for GLI2

This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
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UniProt Comments for GLI2

Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992). Functions as transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor (). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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