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OCLN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2403469

Occludin antibody is an unconjugated rabbit polyclonal antibody to Occludin (OCLN) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.OCLN (Occludin) is an integral membrane protein that is required for cytokine-induced… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
OCLN/Occludin, Polyclonal Antibody
Full Product Name
OCLN/Occludin Rabbit anti-Human Polyclonal Antibody
Product Gene Name
anti-OCLN antibody
Product Synonym Names
PathPlus OCLN/Occludin Antibody; OCLN; Occludin; BLCPMG
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat, Human
Form/Format
PBS containing 0.02% Sodium Azide
Purity/Purification
Peptide Column Affinity Chromatography
Concentration
1mg/ml (lot specific)
Specificity
At least three isoforms of OCLN are known to exist.
Preparation and Storage
Store at 4 degree C for up 3 months. Store at -20 degree C for up to 1 year. Avoid freeze/thaw cycles.
Molecular Weight
65kDa
Chromosome Location
Chromosome: 5; NC_000005.9 (68788119..68850397). Location: 5q13.1
OMIM
251290
3D Structure
ModBase 3D Structure for Q16625
Target
Human OCLN/Occludin
Immunogen
A 15 AA synthetic peptide near the C-Terminus of human OCLN. The immunogen is located within the last 50 AA of OCLN.
Conjugation
Unconjugated
Applications Tested/Suitable for anti-OCLN antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Application Notes for anti-OCLN antibody
IHC-P: 5ug/ml
WB: 1-2ug/ml
OCLN antibody can be used for detection of OCLN by Western blot at 1ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5ug/mL. For immunofluorescence start at 20ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 57 kDa Observed: 57 kDa

Immunocytochemistry (ICC)/Immunofluorescence (IF) of anti-OCLN antibody

OCLN/Occludin Antibody-Immunofluorescence of OCLN in mouse brain tissue with OCLN antibody at 20 ug/mL.
Rabbit Anti-OCLN Polyclonal Antibody – Cat# MBS2403469

Immunocytochemistry (ICC)/Immunofluorescence (IF) of anti-OCLN antibody

OCLN/Occludin Antibody-Immunofluorescence of OCLN in human liver tissue with OCLN antibody at 20 ug/ml.
Rabbit Anti-OCLN Polyclonal Antibody, Immunocytochemistry (ICC)/Immunofluorescence – Cat# MBS2403469

Western Blot (WB) of anti-OCLN antibody

OCLN/Occludin Antibody-Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 ug/mL.
Rabbit Anti-OCLN Polyclonal Antibody, Western Blot (WB) – Cat# MBS2403469

Immunohistochemistry (IHC) of anti-OCLN antibody

OCLN/Occludin Antibody-Human Thryoid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-OCLN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2403469

Product References and Citations for anti-OCLN antibody

The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804

Other Notes

Small volumes of anti-OCLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OCLN purchase

MBS2403469 is an antibody targeting OCLN. Also known as OCLN/Occludin Rabbit anti-Human Polyclonal Antibody. Reactive in Mouse, Rat, Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.

NCBI GI #
4505487
NCBI GeneID
100506658
NCBI Accession #
NP_002529.1
UniProt Primary Accession #
Q16625
UniProt Secondary Accession #
Q8N6K1; B5BU70
UniProt Related Accession #
Q16625; Q5U1V4
GenBank Nucleotide #
NM_002538
NCBI Official Full Name
occludin isoform a
NCBI Official Synonym Full Names
occludin
NCBI Official Symbol
OCLN
NCBI Official Synonym Symbols
BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277
UniProt Protein Name
Occludin
Protein Family
Occludin
UniProt Gene Name
OCLN
UniProt Entry Name
OCLN_HUMAN
NCBI Protein Information
occludin; OTTHUMP00000125187; OTTHUMP00000222347; tight junction protein occludin

UniProt Comments for OCLN

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: tight junction; apicolateral plasma membrane; endocytic vesicle; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cell junction; cytosol

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity

Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis

Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-OCLN antibody

 Reference Product  PubMed Publications
 TJP1 antibody  >3 publications with OCLN and TJP1

Pathways associated with anti-OCLN antibody

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram

Diseases associated with anti-OCLN antibody

 Disease Name  Pubmed Publications
 Hepatitis Antibodies  >9 publications with OCLN and Hepatitis
 Liver Diseases Antibodies  >5 publications with OCLN and Liver Diseases
 Neoplasms Antibodies  >5 publications with OCLN and Neoplasms
 Liver Neoplasms Antibodies  >4 publications with OCLN and Liver Neoplasms
 Leukemia Antibodies  >3 publications with OCLN and Leukemia
 Central Nervous System Diseases Antibodies  >3 publications with OCLN and Central Nervous System Diseases
 Cardiovascular Diseases Antibodies  >2 publications with OCLN and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with OCLN and Carcinoma, Hepatocellular
 Nervous System Malformations Antibodies  >2 publications with OCLN and Nervous System Malformations
 Vascular Diseases Antibodies  >2 publications with OCLN and Vascular Diseases

Organs/Tissues associated with anti-OCLN antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with OCLN and Testis
 Lung Antibodies  >1 publications with OCLN and Lung
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