• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

FEM1B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240353

Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aa and FEM1b (1,2). F1Aa/FEM1b is… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
FEM1B, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human FEM1B
Product Gene Name
anti-FEM1B antibody
Product Synonym Names
Anti-FEM1B Antibody (aa609-622) IHC-plus; FEM1B; F1AA; FEM-1 (C.elegans) homolog b; FEM1-beta; FIAA; KIAA0396; F1A-ALPHA; Fem-1 homolog b (C. elegans); Protein fem-1 homolog B; Human FEM1B
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Bat, Gibbon, Bovine, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Peptide (DINYQDQIPRTLEE) corresponding to amino acids 609 to 622 of human F1Aa This sequence is identical to the corresponding sequence of mouse FEM1b.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
70,264 Da
OMIM
613539
3D Structure
ModBase 3D Structure for Q9UK73
Target Species
Human
Immunogen Description
Synthetic peptide from human FEM1B. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%); Opossum (86%).
Immunogen Type
Synthetic peptide
Immunogen
FEM1B antibody was raised against synthetic peptide from human FEM1B. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%); Opossum (86%).
Antigen Modification
aa609-622
Applications Tested/Suitable for anti-FEM1B antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-FEM1B antibody
IHC-P (10 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-FEM1B antibody

Anti-FEM1B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-FEM1B Polyclonal Antibody – Cat# MBS240353

Immunofluorescence (IF) of anti-FEM1B antibody

Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 ug/ml.
Rabbit Anti-FEM1B Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS240353

Western Blot (WB) of anti-FEM1B antibody

Western blot of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 ug/ml.
Rabbit Anti-FEM1B Polyclonal Antibody, Western Blot (WB) – Cat# MBS240353

Other Notes

Small volumes of anti-FEM1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FEM1B purchase

MBS240353 is an antibody targeting FEM1B. Also known as Rabbit Polyclonal to Human FEM1B. Reactive in Bat, Gibbon, Bovine, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FEM1B. It may not necessarily be applicable to this product.

NCBI GI #
7657265
NCBI GeneID
10116
NCBI Accession #
NP_056137.1
UniProt Primary Accession #
Q9UK73
UniProt Secondary Accession #
O43146
UniProt Related Accession #
Q9UK73
GenBank Nucleotide #
NM_015322.4
NCBI Official Full Name
protein fem-1 homolog B
NCBI Official Synonym Full Names
fem-1 homolog b (C. elegans)
NCBI Official Symbol
FEM1B
NCBI Official Synonym Symbols
F1AA; F1A-ALPHA; FEM1-beta
UniProt Protein Name
Protein fem-1 homolog B
UniProt Synonym Protein Names
FEM1-beta; Fem-1-like death receptor-binding protein alpha; Fem-1-like in apoptotic pathway protein alpha; F1A-alpha
UniProt Gene Name
FEM1B
UniProt Synonym Gene Names
F1AA; KIAA0396; FEM1b; F1A-alpha
UniProt Entry Name
FEM1B_HUMAN
NCBI Protein Information
protein fem-1 homolog B; FEM-1-like death receptor binding protein; fem-1-like in apoptotic pathway protein alpha; fem-1-like death receptor-binding protein alpha

NCBI Summary for FEM1B

This gene encodes an ankyrin repeat protein that belongs to the death receptor-associated family of proteins and plays a role in mediating apoptosis. The encoded protein is also thought to function in the replication stress-induced checkpoint signaling pathway via interaction with checkpoint kinase 1. [provided by RefSeq, Aug 2013]
Show more

UniProt Comments for FEM1B

FEM1B: Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1. Belongs to the fem-1 family.

Protein type: Ligase; Apoptosis; Cell development/differentiation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; death receptor binding; ubiquitin-protein ligase activity

Biological Process: apoptosis; regulation of ubiquitin-protein ligase activity; protein ubiquitination
Show more

Research Articles on FEM1B

1. These findings have implications for understanding the cellular functions of Fem1b, and the regulation of Gli1 oncoprotein activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FEM1B antibody

 Reference Product  PubMed Publications
 GNB2L1 antibody  >1 publications with FEM1B and GNB2L1
 APAF1 antibody  >1 publications with FEM1B and APAF1
 RBX1 antibody  >1 publications with FEM1B and RBX1
 CUL2 antibody  >1 publications with FEM1B and CUL2

Diseases associated with anti-FEM1B antibody

 Disease Name  Pubmed Publications
 Ovarian Diseases Antibodies  >2 publications with FEM1B and Ovarian Diseases
 Necrosis Antibodies  >2 publications with FEM1B and Necrosis
 Brain Diseases Antibodies  >1 publications with FEM1B and Brain Diseases

Organs/Tissues associated with anti-FEM1B antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >5 publications with FEM1B and Testis
 Brain Antibodies  >2 publications with FEM1B and Brain
 Muscle Antibodies  >1 publications with FEM1B and Muscle
 Liver Antibodies  >1 publications with FEM1B and Liver
 Pancreas Antibodies  >1 publications with FEM1B and Pancreas
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000