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DOK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240360

DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with… More details

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Product Overview

Product Name
DOK1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human DOK1
Product Gene Name
anti-DOK1 antibody
Product Synonym Names
Anti-DOK1 Antibody (C-Terminus) IHC-plus; DOK1; Docking protein 1; Gap binding protein p62dok; p62(dok); p62DOK; Pp62; Human DOK1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
peptide corresponding to amino acids near the carboxy terminus of human p62dok
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
37,405 Da
OMIM
602919
3D Structure
ModBase 3D Structure for Q99704
Target Species
Human
Immunogen Description
Synthetic peptide from human DOK1.
Immunogen Type
Synthetic peptide
Immunogen
DOK1 antibody was raised against synthetic peptide from human DOK1.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-DOK1 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-DOK1 antibody
ICC, IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-DOK1 antibody

Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-DOK1 Polyclonal Antibody – Cat# MBS240360

Immunohistochemistry (IHC) of anti-DOK1 antibody

Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-DOK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS240360

Immunofluorescence (IF) of anti-DOK1 antibody

Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Rabbit Anti-DOK1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS240360

Immunocytochemistry (ICC) of anti-DOK1 antibody

Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.
Rabbit Anti-DOK1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS240360

Western Blot (WB) of anti-DOK1 antibody

Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Rabbit Anti-DOK1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS240360

Other Notes

Small volumes of anti-DOK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DOK1 purchase

MBS240360 is an antibody targeting DOK1. Also known as Rabbit Polyclonal to Human DOK1. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DOK1. It may not necessarily be applicable to this product.

NCBI GI #
4503357
NCBI GeneID
1796
NCBI Accession #
NP_001372.1
UniProt Primary Accession #
Q99704
UniProt Secondary Accession #
O43204; Q53TY2; Q9UHG6
UniProt Related Accession #
Q99704
GenBank Nucleotide #
NM_001381.3
NCBI Official Full Name
docking protein 1 isoform 1
NCBI Official Synonym Full Names
docking protein 1, 62kDa (downstream of tyrosine kinase 1)
NCBI Official Symbol
DOK1
NCBI Official Synonym Symbols
P62DOK
UniProt Protein Name
Docking protein 1
UniProt Synonym Protein Names
Downstream of tyrosine kinase 1; p62(dok); pp62
Protein Family
Docking protein
UniProt Gene Name
DOK1
UniProt Entry Name
DOK1_HUMAN
NCBI Protein Information
docking protein 1; pp62; p62(dok); Downstream of tyrosine kinase 1; docking protein 1 (downstream of tyrosine kinase 1)

NCBI Summary for DOK1

The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
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UniProt Comments for DOK1

DOK1: a docking protein that interacts with receptor tyrosine kinases. It is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; receptor signaling protein activity; insulin receptor binding

Biological Process: cell surface receptor linked signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway
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Research Articles on DOK1

1. Taken together, these results reveal that Dok1 and Dok2 proteins are involved in an intrinsic negative feedback loop downstream of natural killer-cell-activating receptors in mouse and human.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DOK1 antibody

 Reference Product  PubMed Publications
 SRC antibody  >61 publications with DOK1 and SRC
 CSK antibody  >9 publications with DOK1 and CSK
 RET antibody  >7 publications with DOK1 and RET
 LCK antibody  >6 publications with DOK1 and LCK
 TEC antibody  >5 publications with DOK1 and TEC
 SHC1 antibody  >4 publications with DOK1 and SHC1
 LYN antibody  >3 publications with DOK1 and LYN
 ABL1 antibody  >2 publications with DOK1 and ABL1

Pathways associated with anti-DOK1 antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies  Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
 IGF1R Signaling Cascade Pathway antibodies  IGF1R Signaling Cascade Pathway Diagram
 IRS Activation Pathway antibodies  IRS Activation Pathway Diagram
 IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 IRS-related Events Pathway antibodies  IRS-related Events Pathway Diagram
 IRS-related Events Triggered By IGF1R Pathway antibodies  IRS-related Events Triggered By IGF1R Pathway Diagram
 Insulin Pathway antibodies  Insulin Pathway Diagram
 Insulin Receptor Signalling Cascade Pathway antibodies  Insulin Receptor Signalling Cascade Pathway Diagram

Diseases associated with anti-DOK1 antibody

 Disease Name  Pubmed Publications
 Leukemia Antibodies  >22 publications with DOK1 and Leukemia
 Neoplasms, Experimental Antibodies  >7 publications with DOK1 and Neoplasms, Experimental
 Lymphoma Antibodies  >6 publications with DOK1 and Lymphoma
 Disease Models, Animal Antibodies  >5 publications with DOK1 and Disease Models, Animal
 Inflammation Antibodies  >4 publications with DOK1 and Inflammation
 Necrosis Antibodies  >4 publications with DOK1 and Necrosis
 Hypertrophy Antibodies  >3 publications with DOK1 and Hypertrophy
 Hyperplasia Antibodies  >2 publications with DOK1 and Hyperplasia
 Breast Neoplasms Antibodies  >2 publications with DOK1 and Breast Neoplasms
 Stomach Neoplasms Antibodies  >2 publications with DOK1 and Stomach Neoplasms

Organs/Tissues associated with anti-DOK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >44 publications with DOK1 and Blood
 Bone Antibodies  >16 publications with DOK1 and Bone
 Bone Marrow Antibodies  >14 publications with DOK1 and Bone Marrow
 Lung Antibodies  >7 publications with DOK1 and Lung
 Muscle Antibodies  >6 publications with DOK1 and Muscle
 Heart Antibodies  >4 publications with DOK1 and Heart
 Embryonic Tissue Antibodies  >4 publications with DOK1 and Embryonic Tissue
 Adipose Tissue Antibodies  >3 publications with DOK1 and Adipose Tissue
 Connective Tissue Antibodies  >3 publications with DOK1 and Connective Tissue
 Spleen Antibodies  >3 publications with DOK1 and Spleen
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