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RYBP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240377

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several… More details

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Product Overview

Product Name
RYBP, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human RYBP
Product Gene Name
Product Synonym Names
Anti-RYBP Antibody (aa215-228) IHC-plus; RYBP; AAP1; APAP-1; Apoptin-associating protein 1; DED-associated factor; DEDAF; Ring1 interactor RYBP; RING1 and YY1 binding protein; YEAF1; RING1 and YY1-binding protein; Human RYBP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
synthetic peptide (TPKGDMSAVNDESF) corresponding to amino acids 215 to 228 of human DEDAF The sequence is identical to that of mouse origin
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
24,822 Da
OMIM
607535
3D Structure
ModBase 3D Structure for Q8N488
Target Species
Human
Immunogen Description
Synthetic peptide from human RYBP. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%); Xenopus (93%).
Immunogen Type
Synthetic peptide
Immunogen
RYBP antibody was raised against synthetic peptide from human RYBP. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%); Xenopus (93%).
Antigen Modification
aa215-228

Product Categories/Family for anti-RYBP antibody

Applications Tested/Suitable for anti-RYBP antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-RYBP antibody
IHC-P (2.5 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-RYBP antibody

Anti-RYBP antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-RYBP Polyclonal Antibody – Cat# MBS240377

Immunohistochemistry (IHC) of anti-RYBP antibody

Anti-RYBP antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-RYBP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS240377

Immunofluorescence (IF) of anti-RYBP antibody

Immunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 ug/ml.
Rabbit Anti-RYBP Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS240377

Western Blot (WB) of anti-RYBP antibody

Western blot of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 ug /ml.
Rabbit Anti-RYBP Polyclonal Antibody, Western Blot (WB) – Cat# MBS240377

Other Notes

Small volumes of anti-RYBP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RYBP purchase

MBS240377 is an antibody targeting RYBP. Also known as Rabbit Polyclonal to Human RYBP. Reactive in Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RYBP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RING1 and YY1-binding protein
NCBI Official Synonym Full Names
RING1 and YY1 binding protein
NCBI Official Symbol
RYBP
NCBI Official Synonym Symbols
AAP1; DEDAF; YEAF1
UniProt Protein Name
RING1 and YY1-binding protein
UniProt Synonym Protein Names
Apoptin-associating protein 1; APAP-1; Death effector domain-associated factor; DED-associated factor; YY1 and E4TF1-associated factor 1
UniProt Gene Name
RYBP
UniProt Synonym Gene Names
DEDAF; YEAF1; APAP-1; DED-associated factor
UniProt Entry Name
RYBP_HUMAN
NCBI Protein Information
RING1 and YY1-binding protein; APAP-1; DED-associated factor; ring1 interactor RYBP; apoptin-associating protein 1; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1; death effector domain-associated factor

UniProt Comments for RYBP

RYBP: Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: nucleoplasm; cytoplasm; PcG protein complex

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; apoptosis; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RYBP antibody

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