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PDCD1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240383

PD-1 (PDCD1) encodes a cell surface membrane protein of the immunoglobulin superfamily. PD-1 is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies… More details

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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDCD1 / CD279 / PD-1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human PDCD1 / CD279 / PD-1
Product Gene Name
anti-PDCD1 antibody
Product Synonym Gene Name
CD279; PD-1
Product Synonym Names
Anti-PDCD1 / CD279 / PD-1 Antibody (C-Terminus) IHC-plus; PDCD1; CD279; HPD-1; HPD-l; PD1; Protein PD-1; SLEB2; PD-1; CD279 antigen; Programmed cell death 1; Human PDCD1; CD279; PD-1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Peptide Column Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for up 3 months. Store at -20 degree C for up to 1 yr. Avoid freeze/thaw cycles.
Molecular Weight
31,647 Da
OMIM
gene 605218
Target Species
Human
Immunogen Type
Synthetic peptide
Immunogen
PDCD1 / CD279 / PD-1 antibody was raised against synthetic peptide from human PDCD1 / CD279.
Antigen Modification
C-Terminus
Target
Human PDCD1/CD279/PD-1
Family/Subfamily
Apoptosis
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-PDCD1 antibody

Family: Apoptosis
Applications Tested/Suitable for anti-PDCD1 antibody
Immunohistochemistry (IHC - Paraffin); Immunofluorescence (IF); Western blot (WB); ELISA
Application Notes for anti-PDCD1 antibody
IHC-P (2.5 ug/ml)
Usage: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 45 kDa

Immunohistochemistry (IHC) of anti-PDCD1 antibody

Anti-PD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-PDCD1 Polyclonal Antibody – Cat# MBS240383

Immunofluorescence (IF) of anti-PDCD1 antibody

Immunofluorescence of PD-1 in Human Brain cells with PD-1 antibody at 20 ug/ml.
Rabbit Anti-PDCD1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS240383

Western Blot (WB) of anti-PDCD1 antibody

Western blot of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
Rabbit Anti-PDCD1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS240383

Other Notes

Small volumes of anti-PDCD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDCD1 purchase

MBS240383 is an antibody targeting PDCD1. Also known as Rabbit Polyclonal to Human PDCD1 / CD279 / PD-1. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDCD1. It may not necessarily be applicable to this product.

NCBI GI #
167857792
NCBI GeneID
5133
NCBI Accession #
NP_005009.2
UniProt Secondary Accession #
O00517; Q8IX89
UniProt Related Accession #
Q15116
GenBank Nucleotide #
NM_005018.2
NCBI Official Full Name
programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
UniProt Protein Name
Programmed cell death protein 1
UniProt Synonym Protein Names
CD_antigen: CD279
Protein Family
Programmed cell death protein
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1
UniProt Entry Name
PDCD1_HUMAN
NCBI Protein Information
programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2

NCBI Summary for PDCD1

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
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UniProt Comments for PDCD1

PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: signal transducer activity; protein binding

Biological Process: apoptosis; multicellular organismal development; T cell costimulation; signal transduction; humoral immune response

Disease: Systemic Lupus Erythematosus
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Research Articles on PDCD1

1. The results demonstrate for the first time that the PD-1 rs10204525 polymorphism might contribute to susceptibility of ESCC

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDCD1 antibody

 Reference Product  PubMed Publications
 CD4 antibody  >410 publications with PDCD1 and CD4
 CD274 antibody  >329 publications with PDCD1 and CD274
 PDCD1LG2 antibody  >97 publications with PDCD1 and PDCD1LG2
 PTPN6 antibody  >7 publications with PDCD1 and PTPN6
 PTPN11 antibody  >7 publications with PDCD1 and PTPN11
 HLA-DR4 antibody  >1 publications with PDCD1 and HLA-DR4

Pathways associated with anti-PDCD1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 PD-1 Signaling Pathway antibodies  PD-1 Signaling Pathway Diagram
 T Cell Receptor Signaling Pathway antibodies  T Cell Receptor Signaling Pathway Diagram
 T Cell Receptor Signaling Pathway antibodies  T Cell Receptor Signaling Pathway Diagram

Diseases associated with anti-PDCD1 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >146 publications with PDCD1 and Liver Diseases
 Inflammation Antibodies  >115 publications with PDCD1 and Inflammation
 Melanoma Antibodies  >74 publications with PDCD1 and Melanoma
 Necrosis Antibodies  >73 publications with PDCD1 and Necrosis
 Lupus Erythematosus, Systemic Antibodies  >72 publications with PDCD1 and Lupus Erythematosus, Systemic
 Lung Diseases Antibodies  >57 publications with PDCD1 and Lung Diseases
 Adenocarcinoma Antibodies  >35 publications with PDCD1 and Adenocarcinoma
 Kidney Diseases Antibodies  >28 publications with PDCD1 and Kidney Diseases
 Liver Neoplasms Antibodies  >26 publications with PDCD1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >20 publications with PDCD1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-PDCD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >931 publications with PDCD1 and Blood
 Kidney Antibodies  >36 publications with PDCD1 and Kidney
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