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IPAF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240391

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another… More details

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Product Overview

Online Datasheet in PDF
Product Name
IPAF / NLRC4, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human IPAF / NLRC4
Product Gene Name
anti-IPAF antibody
Product Synonym Gene Name
NLRC4
Product Synonym Names
Anti-IPAF / NLRC4 Antibody (C-Terminus) IHC-plus; NLRC4; CARD12; CLANA; CLANB; CLANC; CLAND; CLAN; CLR2.1; Clan protein; CLAN1; Ice protease-activating factor; NOD-like receptor C4; ICE-protease activating factor; IPAF; Human IPAF; NLRC4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
10,646 Da
OMIM
606831
3D Structure
ModBase 3D Structure for Q9NPP4
Target Species
Human
Immunogen Description
Synthetic peptide from human NLRC4 / IPAF.
Immunogen Type
Synthetic peptide
Immunogen
IPAF / NLRC4 antibody was raised against synthetic peptide from human NLRC4 / IPAF.
Antigen Modification
C-Terminus

Product Categories/Family for anti-IPAF antibody

Family: Apoptosis
Applications Tested/Suitable for anti-IPAF antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-IPAF antibody
ICC, IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-IPAF antibody

Anti-NLRC4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-IPAF Polyclonal Antibody – Cat# MBS240391

Immunofluorescence (IF) of anti-IPAF antibody

Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 ug/ml.
Rabbit Anti-IPAF Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS240391

Immunocytochemistry (ICC) of anti-IPAF antibody

Immunocytochemistry of Ipaf in THP-1 cells with Ipaf antibody at 10 ug/ml.
Rabbit Anti-IPAF Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS240391

Western Blot (WB) of anti-IPAF antibody

Western blot of Ipaf in human PBL lysate with Ipaf antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) ug/ml, respectively.
Rabbit Anti-IPAF Polyclonal Antibody, Western Blot (WB) – Cat# MBS240391

Other Notes

Small volumes of anti-IPAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IPAF purchase

MBS240391 is an antibody targeting IPAF. Also known as Rabbit Polyclonal to Human IPAF / NLRC4. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IPAF. It may not necessarily be applicable to this product.

NCBI GI #
40788015
NCBI GeneID
58484
NCBI Accession #
NP_067032.3
UniProt Primary Accession #
Q9NPP4
UniProt Secondary Accession #
Q96J81; Q96J82; Q96J83; A8K9F8; B2RBQ3; B3KTF0; D6W580
UniProt Related Accession #
Q9NPP4
GenBank Nucleotide #
NM_021209.4
NCBI Official Full Name
NLR family CARD domain-containing protein 4 isoform a
NCBI Official Synonym Full Names
NLR family, CARD domain containing 4
NCBI Official Symbol
NLRC4
NCBI Official Synonym Symbols
CLAN; IPAF; AIFEC; CLAN1; CLANA; CLANB; CLANC; CLAND; FCAS4; CARD12; CLR2.1
UniProt Protein Name
NLR family CARD domain-containing protein 4
UniProt Synonym Protein Names
CARD, LRR, and NACHT-containing protein; Clan protein; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; Ipaf
UniProt Gene Name
NLRC4
UniProt Synonym Gene Names
CARD12; CLAN; CLAN1; IPAF; Clan protein; Ipaf
UniProt Entry Name
NLRC4_HUMAN
NCBI Protein Information
NLR family CARD domain-containing protein 4; NOD-like receptor C4; ICE-protease activating factor; CARD, LRR, and NACHT-containing protein; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4

NCBI Summary for IPAF

This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
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UniProt Comments for IPAF

NLRC4: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2p22.3

Cellular Component: spindle microtubule; cytoplasm; intracellular; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; ubiquitin-protein ligase activity; magnesium ion binding; cysteine protease inhibitor activity; ATP binding

Biological Process: caspase activation; detection of bacterium; positive regulation of apoptosis; protein ubiquitination; regulation of signal transduction; activation of NF-kappaB transcription factor; regulation of neuron apoptosis; defense response to bacterium; innate immune response; interleukin-1 beta secretion; inflammatory response; activation of innate immune response; protein homooligomerization; negative regulation of apoptosis

Disease: Autoinflammation With Infantile Enterocolitis; Familial Cold Autoinflammatory Syndrome 4
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Research Articles on IPAF

1. Data show that the disease was caused by a de novo gain-of-function mutation in NLR family CARD domain containing 4 (NLRC4) encoding a p.Val341Ala substitution.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IPAF antibody

 Reference Product  PubMed Publications
 CASP1 antibody  >171 publications with IPAF and CASP1
 NLRP3 antibody  >121 publications with IPAF and NLRP3
 NLRP1 antibody  >35 publications with IPAF and NLRP1
 PYCARD antibody  >26 publications with IPAF and PYCARD
 NAIP antibody  >23 publications with IPAF and NAIP
 NOD2 antibody  >15 publications with IPAF and NOD2
 NOD1 antibody  >12 publications with IPAF and NOD1
 NLRP4 antibody  >4 publications with IPAF and NLRP4

Pathways associated with anti-IPAF antibody

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 NOD Pathway antibodies  NOD Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways antibodies  Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways Diagram

Diseases associated with anti-IPAF antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >94 publications with IPAF and Inflammation
 Immune System Diseases Antibodies  >20 publications with IPAF and Immune System Diseases
 Necrosis Antibodies  >17 publications with IPAF and Necrosis
 Pneumonia Antibodies  >12 publications with IPAF and Pneumonia
 Lung Diseases Antibodies  >10 publications with IPAF and Lung Diseases
 Disease Models, Animal Antibodies  >8 publications with IPAF and Disease Models, Animal
 Liver Diseases Antibodies  >3 publications with IPAF and Liver Diseases
 Nervous System Diseases Antibodies  >3 publications with IPAF and Nervous System Diseases
 Enterocolitis Antibodies  >3 publications with IPAF and Enterocolitis
 Macrophage Activation Syndrome Antibodies  >2 publications with IPAF and Macrophage Activation Syndrome

Organs/Tissues associated with anti-IPAF antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >32 publications with IPAF and Blood
 Bone Marrow Antibodies  >27 publications with IPAF and Bone Marrow
 Liver Antibodies  >8 publications with IPAF and Liver
 Brain Antibodies  >5 publications with IPAF and Brain
 Thymus Antibodies  >2 publications with IPAF and Thymus
 Kidney Antibodies  >2 publications with IPAF and Kidney
 Uterus Antibodies  >1 publications with IPAF and Uterus
 Testis Antibodies  >1 publications with IPAF and Testis
 Bladder Antibodies  >1 publications with IPAF and Bladder
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