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SPANXC Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240445

Sperm Protein Associated with the Nucleus, X Chromosome, family member C (SPANX-C) was originally isolated in a search for genes that contribute to the metastatic pathway of neoplastic cells. Expression in melanoma cells was found in only the most aggressive,… More details

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Product Overview

Product Name
SPANXC / SPANX-C, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human SPANXC / SPANX-C
Product Gene Name
anti-SPANXC antibody
Product Synonym Gene Name
SPANX-C
Product Synonym Names
Anti-SPANXC / SPANX-C Antibody IHC-plus; SPANXC; CTp11; CT11.3; SPANX family member C; SPAN-Xc protein; SPANX family; member C; SPANX-C; Cancer/testis antigen 11.3; Human SPANXC; SPANX-C
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Specificity
Full-length recombinant human SPANX-C protein.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
11,002 Da
OMIM
300330
3D Structure
ModBase 3D Structure for Q9NY87
Target Species
Human
Immunogen Description
Recombinant human SPANXC.
Immunogen Type
Recombinant protein
Immunogen
SPANXC / SPANX-C antibody was raised against recombinant human SPANXC.
Applications Tested/Suitable for anti-SPANXC antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SPANXC antibody
ELISA (1:5000 - 1:20000), IHC-P (2.5 ug/ml), WB (1:1000 - 1:5000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-SPANXC antibody

Anti-SPANXC antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-SPANXC Polyclonal Antibody – Cat# MBS240445

Western Blot (WB) of anti-SPANXC antibody

Anti-SPANX-C Antibody - Western Blot. Western blot of Protein A purified anti-SPANX-C antibody shows detection of a band at ~17 kD corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
Rabbit Anti-SPANXC Polyclonal Antibody, Western Blot (WB) – Cat# MBS240445

Other Notes

Small volumes of anti-SPANXC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPANXC purchase

MBS240445 is an antibody targeting SPANXC. Also known as Rabbit Polyclonal to Human SPANXC / SPANX-C. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPANXC. It may not necessarily be applicable to this product.

NCBI GI #
13435137
NCBI GeneID
64663
NCBI Accession #
NP_073152.1
UniProt Primary Accession #
Q9NY87
UniProt Secondary Accession #
Q32WL9; Q5JX88
UniProt Related Accession #
Q9NY87
GenBank Nucleotide #
NM_022661.2
NCBI Official Full Name
sperm protein associated with the nucleus on the X chromosome C
NCBI Official Synonym Full Names
SPANX family, member C
NCBI Official Symbol
SPANXC
NCBI Official Synonym Symbols
C; CTp11; CT11.3; SPANX-C
UniProt Protein Name
Sperm protein associated with the nucleus on the X chromosome C
UniProt Synonym Protein Names
Cancer/testis antigen 11.3; CT11.3; Cancer/testis-associated protein CTp11; Nuclear-associated protein SPAN-Xc; SPANX-C; SPANX family member C
Protein Family
Sperm protein associated with the nucleus on the X chromosome
UniProt Gene Name
SPANXC
UniProt Synonym Gene Names
CT11.3; SPANX-C
UniProt Entry Name
SPNXC_HUMAN
NCBI Protein Information
sperm protein associated with the nucleus on the X chromosome C; SPAN-Xc protein; cancer/testis antigen 11.3; nuclear-associated protein SPAN-Xc; cancer-testis-associated protein CTp11; cancer/testis-associated protein CTp11; cancer/testis antigen family 11, member 3; cancer/testis-associated protein of 11 kD; sperm protein associated with the nucleus, X chromosome, family member C

NCBI Summary for SPANXC

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family, which is located in a gene cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a protein that localizes to the nucleus and is expressed in highly metastatic cell lines, making the protein a potential diagnostic and prognostic marker. The protein belongs to a family of cancer/testis antigens and represents a potential target for cancer immunotherapy. [provided by RefSeq, Jul 2008]
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UniProt Comments for SPANXC

SPANXC: Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family, which is located in a gene cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a protein that localizes to the nucleus and is expressed in highly metastatic cell lines, making the protein a potential diagnostic and prognostic marker. The protein belongs to a family of cancer/testis antigens and represents a potential target for cancer immunotherapy. [provided by RefSeq, Jul 2008]

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: cytoplasm; nucleus
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Research Articles on SPANXC

1. study reports the isolation of AKAP3 and CTp11 Cancer/testis antigens from hepatocellular carcinoma patient sera

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPANXC antibody

 Reference Product  PubMed Publications
 LDOC1 antibody  >2 publications with SPANXC and LDOC1

Organs/Tissues associated with anti-SPANXC antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >8 publications with SPANXC and Testis
 Liver Antibodies  >2 publications with SPANXC and Liver
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