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PHAP III Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240491

Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to… More details

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Sample Manual Insert View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
PHAP III / ANP32E, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human PHAP III / ANP32E
Product Gene Name
Product Synonym Gene Name
ANP32E
Product Synonym Names
Anti-PHAP III / ANP32E Antibody (C-Terminus) IHC-plus; ANP32E; LANP-L; LANPL; LANP-like protein; Human PHAP III; ANP32E
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
25,125 Da
OMIM
609611
3D Structure
ModBase 3D Structure for Q9BTT0
Target Species
Human
Immunogen Description
Synthetic peptide from human ANP32E.
Immunogen Type
Synthetic peptide
Immunogen
PHAP III / ANP32E antibody was raised against synthetic peptide from human ANP32E.
Antigen Modification
C-Terminus

Product Categories/Family for anti-PHAP III antibody

Applications Tested/Suitable for anti-PHAP III antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-PHAP III antibody
IHC-P (2.5 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-PHAP III antibody

Anti-ANP32E antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-PHAP III Polyclonal Antibody – Cat# MBS240491

Immunofluorescence (IF) of anti-PHAP III antibody

Immunofluorescence of PHAP III in Human Small Intestine cells with PHAP III antibody at 10 ug/ml.
Rabbit Anti-PHAP III Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS240491

Western Blot (WB) of anti-PHAP III antibody

Western blot of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with PHAP antibody III at 1 ug/ml.
Rabbit Anti-PHAP III Polyclonal Antibody, Western Blot (WB) – Cat# MBS240491

Other Notes

Small volumes of anti-PHAP III antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHAP III purchase

MBS240491 is an antibody targeting PHAP III. Also known as Rabbit Polyclonal to Human PHAP III / ANP32E. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PHAP III. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member E isoform 1
NCBI Official Synonym Full Names
acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
NCBI Official Symbol
ANP32E
NCBI Official Synonym Symbols
LANPL; LANP-L
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member E
UniProt Synonym Protein Names
LANP-like protein; LANP-L
UniProt Gene Name
ANP32E
UniProt Synonym Gene Names
LANP-L
UniProt Entry Name
AN32E_HUMAN
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member E; LANP-like protein; leucine-rich acidic nuclear protein like

UniProt Comments for PHAP III

LANP-L: Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity. Belongs to the ANP32 family.

Protein type: Inhibitor; Vesicle; Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasmic membrane-bound vesicle; nucleus

Molecular Function: phosphatase inhibitor activity; histone binding

Biological Process: negative regulation of catalytic activity; histone exchange
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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