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RAD52 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240523

MBS240523 is an antibody targeting RAD52. Also known as Rabbit Polyclonal to Human RAD52. Reactive in Human. Host: Rabbit. More details

$715 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAD52, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human RAD52
Product Gene Name
Product Synonym Names
Anti-RAD52 Antibody (aa360-375) IHC-plus; RAD52; RAD52 (S. cerevisiae) homolog; RAD52 homolog (S. cerevisiae); Recombination protein RAD52; Human RAD52
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
aa 360 - 375 of the Human Rad 52 protein.
Preparation and Storage
Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.
Molecular Weight
12,943 Da
OMIM
600392
3D Structure
ModBase 3D Structure for P43351
Target Species
Human
Immunogen Description
Synthetic peptide from human RAD52.
Immunogen Type
Synthetic peptide
Immunogen
RAD52 antibody was raised against synthetic peptide from human RAD52.
Antigen Modification
aa360-375
Applications Tested/Suitable for anti-RAD52 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RAD52 antibody
ELISA (1:5000 - 1:20000), IHC-P (2.5 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-RAD52 antibody

Anti-RAD52 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-RAD52 Polyclonal Antibody – Cat# MBS240523

Western Blot (WB) of anti-RAD52 antibody

Anti-Human RAD52 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~63.0 kD is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] (diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by.
Rabbit Anti-RAD52 Polyclonal Antibody, Western Blot (WB) – Cat# MBS240523

Other Notes

Small volumes of anti-RAD52 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAD52. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA repair protein RAD52 homolog isoform a
NCBI Official Synonym Full Names
RAD52 homolog (S. cerevisiae)
NCBI Official Symbol
RAD52
UniProt Protein Name
DNA repair protein RAD52 homolog
UniProt Gene Name
RAD52
UniProt Entry Name
RAD52_HUMAN
NCBI Protein Information
DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23

NCBI Summary for RAD52

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]
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UniProt Comments for RAD52

RAD52: a protein involved in DNA double strand breaks and recombination. Associates with and is phosphorylated by c-Abl. Belongs to the RAD52/RAD22 family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 12p13-p12.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding

Biological Process: DNA recombinase assembly; double-strand break repair; DNA repair; double-strand break repair via homologous recombination; DNA recombination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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