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PPP1R13L Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240529

MBS240529 is an antibody targeting PPP1R13L. Also known as Rabbit Polyclonal to Human PPP1R13L / RAI / IASPP. Reactive in Human. Host: Rabbit. More details

$715 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PPP1R13L / RAI / IASPP, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human PPP1R13L / RAI / IASPP
Product Gene Name
anti-PPP1R13L antibody
Product Synonym Gene Name
RAI; IASPP
Product Synonym Names
Anti-PPP1R13L / RAI / IASPP Antibody (aa780-797) IHC-plus; PPP1R13L; IASPP; Inhibitor of ASPP protein; NFkB-interacting protein 1; NKIP1; RAI4; RAI; Retinoic acid induced 4; NFkB interacting protein 1; PPP1R13BL; Protein iASPP; RelA-associated inhibitor; PPP1R13B-like protein; Human PPP1R13L; RAI; IASPP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Amino acids 780-797 of human iASPP (isoform 1) protein.
Preparation and Storage
Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.
Molecular Weight
89,091 Da
OMIM
607463
3D Structure
ModBase 3D Structure for Q8WUF5
Target Species
Human
Immunogen Description
Synthetic peptide from human PPP1R13L / IASPP.
Immunogen Type
Synthetic peptide
Immunogen
PPP1R13L / RAI / IASPP antibody was raised against synthetic peptide from human PPP1R13L / IASPP.
Antigen Modification
aa780-797
Applications Tested/Suitable for anti-PPP1R13L antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PPP1R13L antibody
ELISA (1:40000 - 1:160000), IF, IHC-P (2.5 ug/ml), WB (1:1000 - 1:5000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-PPP1R13L antibody

Anti-PPP1R13L / IASPP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-PPP1R13L Polyclonal Antibody – Cat# MBS240529

Western Blot (WB) of anti-PPP1R13L antibody

Anti-iASPP Antibody - Western Blot. Western blot of affinity purified anti-iASPP antibody shows detection of a band at ~100 kD (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1500 in 5% BLOTTO/PBS overnight at 4C. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Rabbit Anti-PPP1R13L Polyclonal Antibody, Western Blot (WB) – Cat# MBS240529

Other Notes

Small volumes of anti-PPP1R13L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP1R13L. It may not necessarily be applicable to this product.

NCBI GI #
63003907
NCBI GeneID
10848
NCBI Accession #
NP_006654.2
UniProt Primary Accession #
Q8WUF5
UniProt Secondary Accession #
Q2PNZ9; Q5DU71; Q5I1X4; Q6P1R7; Q6PKF8; Q9Y290
UniProt Related Accession #
Q8WUF5
GenBank Nucleotide #
NM_006663.3
NCBI Official Full Name
relA-associated inhibitor
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 13 like
NCBI Official Symbol
PPP1R13L
NCBI Official Synonym Symbols
RAI; RAI4; IASPP; NKIP1
UniProt Protein Name
RelA-associated inhibitor
UniProt Synonym Protein Names
Inhibitor of ASPP protein; Protein iASPP; NFkB-interacting protein 1; PPP1R13B-like protein
Protein Family
RelA-associated inhibitor
UniProt Gene Name
PPP1R13L
UniProt Synonym Gene Names
IASPP; NKIP1; PPP1R13BL; RAI; Protein iASPP
UniProt Entry Name
IASPP_HUMAN
NCBI Protein Information
relA-associated inhibitor; protein iASPP; PPP1R13B-like protein; retinoic acid induced 4; inhibitor of ASPP protein; NFkB interacting protein 1; NFkB-interacting protein 1; inhibitor of apoptosis stimulating protein of p53; protein phosphatase 1, regulatory (inhibitor) subunit 13 like

NCBI Summary for PPP1R13L

IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.[supplied by OMIM, Mar 2008]

UniProt Comments for PPP1R13L

PPP1R13L: an oncoprotein that interacts with and inhibits NF-kappaB, Sp1 and p53. Plays a central role in regulation of apoptosis and transcription. May inhibit p53 by preventing its association with ASPP1 or ASPP2, thereby suppressing the subsequent activation of apoptosis. Required for neuronal survival after axonal injury. Two alternatively spliced isoforms have been described. Isoform 1 is preferrentially found in the cytoplasm; isoform 2 is nuclear.

Protein type: Phosphatase, regulatory subunit; Apoptosis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: intercellular bridge; cytoplasm; cell junction; nucleus

Molecular Function: identical protein binding; protein binding; transcription factor binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; apoptosis; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; multicellular organismal homeostasis; embryonic camera-type eye development; post-embryonic development; cardiac muscle contraction; hair cycle
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Research Articles on PPP1R13L

1. Hematopoietic cells can be protected against apoptosis by iASPP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPP1R13L antibody

 Reference Product  PubMed Publications
 CD3EAP antibody  >15 publications with PPP1R13L and CD3EAP
 RELA antibody  >12 publications with PPP1R13L and RELA
 TP53 antibody  >10 publications with PPP1R13L and TP53
 TP63 antibody  >3 publications with PPP1R13L and TP63
 SP1 antibody  >2 publications with PPP1R13L and SP1

Pathways associated with anti-PPP1R13L antibody

 Products by Pathway  Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram
 P53 Pathway antibodies  P53 Pathway Diagram

Diseases associated with anti-PPP1R13L antibody

 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >16 publications with PPP1R13L and Lung Diseases
 Lung Neoplasms Antibodies  >15 publications with PPP1R13L and Lung Neoplasms
 Breast Neoplasms Antibodies  >10 publications with PPP1R13L and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >6 publications with PPP1R13L and Neoplasm Metastasis
 Leukemia Antibodies  >6 publications with PPP1R13L and Leukemia
 Cell Transformation, Neoplastic Antibodies  >5 publications with PPP1R13L and Cell Transformation, Neoplastic
 Nervous System Diseases Antibodies  >5 publications with PPP1R13L and Nervous System Diseases
 Liver Diseases Antibodies  >4 publications with PPP1R13L and Liver Diseases
 Congenital Abnormalities Antibodies  >3 publications with PPP1R13L and Congenital Abnormalities
 Disease Models, Animal Antibodies  >3 publications with PPP1R13L and Disease Models, Animal

Organs/Tissues associated with anti-PPP1R13L antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >17 publications with PPP1R13L and Lung
 Skin Antibodies  >7 publications with PPP1R13L and Skin
 Liver Antibodies  >4 publications with PPP1R13L and Liver
 Placenta Antibodies  >3 publications with PPP1R13L and Placenta
 Prostate Antibodies  >3 publications with PPP1R13L and Prostate
 Heart Antibodies  >3 publications with PPP1R13L and Heart
 Embryonic Tissue Antibodies  >3 publications with PPP1R13L and Embryonic Tissue
 Brain Antibodies  >2 publications with PPP1R13L and Brain
 Stomach Antibodies  >2 publications with PPP1R13L and Stomach
 Bone Antibodies  >2 publications with PPP1R13L and Bone
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