Rabbit Anti-PNKP Polyclonal Antibody – Cat# MBS9603176

Western blot analysis of PNKP in lysates of Jurkat , using PNKP Antibody(MBS9603176).

PNKP Antibody – Rabbit Polyclonal

Name: PNKP Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS9603176
Price: 325 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9603176

Function: Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension… More details

$325 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PNKP, Polyclonal Antibody

Full Product Name

PNKP Antibody

Product Gene Name

anti-PNKP antibody

Product Synonym Names

2''(3'')-polynucleotidase; 2'(3')-polynucleotidase; Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; DNA 5' kinase/3' phosphatase; DNA 5''-kinase/3''-phosphatase; EIEE10; Homo sapiens polynucleotide kinase 3' phosphatase (PNKP); MCSZ; PNK 1; PNK1; Pnkp; PNKP DNA kinase; PNKP_HUMAN; Polynucleotide 3'-phosphatase; Polynucleotide 5' hydroxyl kinase; Polynucleotide 5''-hydroxyl-kinase; Polynucleotide kinase 3 prime phosphatase; Polynucleotide kinase 3' phosphatase; Polynucleotide Kinase; Polynucleotide kinase-3''-phosphatase

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Form/Format

Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Concentration

1mg/ml (lot specific)

Specificity

PNKP antibody detects endogenous levels of PNKP

Preparation and Storage

Store at -20 degree C. Stable for 12 months from date of receipt.

Antibody/Peptide Pairs

PNKP peptide (MBS9620653) is used for blocking the activity of PNKP antibody (MBS9603176)

Molecular Weight

Observed: 60 kDa
Predicted: 58 kDa

3D Structure

ModBase 3D Structure for Q96T60

Supply Chain Verification

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Immunogen

A synthesized peptide derived from human PNKP

Subcellular Location

Nucleus.

Tissue Specificity

Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).

Conjugation

Unconjugated

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-PNKP antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-PNKP antibody

WB: 1:500-1:2000

Other Notes

Small volumes of anti-PNKP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PNKP purchase

MBS9603176 is an antibody targeting PNKP. Also known as PNKP Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PNKP. It may not necessarily be applicable to this product.

NCBI GI #

31543419

NCBI GeneID

11284

NCBI Accession #

NP_009185.2

UniProt Primary Accession #

Q96T60

UniProt Secondary Accession #

Q9BUL2; Q9P1V2; Q9UKU8; Q9UNF8; Q9UNI0

UniProt Related Accession #

Q96T60

GenBank Nucleotide #

NM_007254.3

NCBI Official Full Name

bifunctional polynucleotide phosphatase/kinase

NCBI Official Synonym Full Names

polynucleotide kinase 3'-phosphatase

NCBI Official Symbol

PNKP

NCBI Official Synonym Symbols

PNK; AOA4; MCSZ; EIEE10

UniProt Protein Name

Bifunctional polynucleotide phosphatase/kinase

UniProt Synonym Protein Names

DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphataseIncluding the following 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32)Alternative name(s):2'(3')-polynucleotidase

UniProt Gene Name

PNKP

NCBI Protein Information

bifunctional polynucleotide phosphatase/kinase

NCBI Summary for PNKP

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]

UniProt Comments for PNKP

Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.

Research Articles on PNKP

1. Despite presence of an alternative 3'-phosphatase, loss of PNKP significantly sensitizes cells to 3'-phosphate-terminated DSBs, due to a 3'-dephosphorylation defect.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PNKP antibody

Reference Product	PubMed Publications
XRCC1 antibody	>13 publications with PNKP and XRCC1
ATM antibody	>5 publications with PNKP and ATM
XRCC4 antibody	>5 publications with PNKP and XRCC4
LIG3 antibody	>2 publications with PNKP and LIG3
LIG4 antibody	>1 publications with PNKP and LIG4

Diseases associated with anti-PNKP antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>9 publications with PNKP and Nervous System Diseases
Microcephaly Antibodies	>7 publications with PNKP and Microcephaly
Seizures Antibodies	>7 publications with PNKP and Seizures
Breast Neoplasms Antibodies	>2 publications with PNKP and Breast Neoplasms
Mitochondrial Diseases Antibodies	>2 publications with PNKP and Mitochondrial Diseases
Developmental Disabilities Antibodies	>2 publications with PNKP and Developmental Disabilities
DNA Repair-Deficiency Disorders Antibodies	>1 publications with PNKP and DNA Repair-Deficiency Disorders

Organs/Tissues associated with anti-PNKP antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>4 publications with PNKP and Lung
Brain Antibodies	>3 publications with PNKP and Brain
Eye Antibodies	>2 publications with PNKP and Eye
Prostate Antibodies	>2 publications with PNKP and Prostate
Skin Antibodies	>1 publications with PNKP and Skin