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EMI2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS240573

MBS240573 is an antibody targeting EMI2. Also known as Rabbit Polyclonal to Human EMI2 / FBXO43. Reactive in Chimpanzee, Human, Mouse, Rat. Host: Rabbit. More details

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Technical Datasheet View PDF
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Product Overview

Product Name
EMI2 / FBXO43, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human EMI2 / FBXO43
Product Gene Name
Product Synonym Gene Name
FBXO43
Product Synonym Names
Anti-EMI2 / FBXO43 Antibody (N-Terminus) IHC-plus; FBXO43; Early mitotic inhibitor 2; EMI2; Endogenous meiotic inhibitor 2; F-box protein 43; ERP1; F-box only protein 43; FBX43; Human EMI2; FBXO43
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Chimpanzee, Human, Mouse, Rat
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
A region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein.
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Molecular Weight
78,402 Da
OMIM
609110
3D Structure
ModBase 3D Structure for Q4G163
Target Species
Human
Immunogen Description
Synthetic peptide from human FBXO43 / EMI2.
Immunogen Type
Synthetic peptide
Immunogen
EMI2 / FBXO43 antibody was raised against synthetic peptide from human FBXO43 / EMI2.
Antigen Modification
N-Terminus

Product Categories/Family for anti-EMI2 antibody

Applications Tested/Suitable for anti-EMI2 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-EMI2 antibody
ELISA (1:10000 - 1:50000), IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:3000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-EMI2 antibody

Anti-FBXO43 / EMI2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-EMI2 Polyclonal Antibody – Cat# MBS240573

Western Blot (WB) of anti-EMI2 antibody

Anti-Fbp5B Antibody - Western Blot. Western blot of affinity purified anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This pre-incubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown). Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.
Rabbit Anti-EMI2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS240573

Other Notes

Small volumes of anti-EMI2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EMI2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
F-box only protein 43
NCBI Official Synonym Full Names
F-box protein 43
NCBI Official Symbol
FBXO43
NCBI Official Synonym Symbols
EMI2; ERP1; FBX43
UniProt Protein Name
F-box only protein 43
UniProt Synonym Protein Names
Endogenous meiotic inhibitor 2
Protein Family
UniProt Gene Name
FBXO43
UniProt Synonym Gene Names
EMI2
UniProt Entry Name
FBX43_HUMAN
NCBI Protein Information
F-box only protein 43; early mitotic inhibitor 2; endogenous meiotic inhibitor 2

NCBI Summary for EMI2

Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
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UniProt Comments for EMI2

FBX43: Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (Probable). Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with CDC20.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: nucleus

Molecular Function: zinc ion binding

Biological Process: meiotic cell cycle; negative regulation of meiosis; protein ubiquitination; negative regulation of protein catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-EMI2 antibody

Organs/Tissues associated with anti-EMI2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with EMI2 and Blood
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