Anti-HDAC1 antibody IHC of human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-HDAC-1 polyclonal antibody-Immunofluorescence Microscopy. polyclonal Anti-HDAC-1 antibody () was used with Atto 425 Anti-Rabbit IgG (shown in red) for to detect HDAC-1 by immunofluorescence. Anti-Keratin monoclonal antibody was used with Dylight 488 goat anti-mouse shown in green) to detect Keratin by Immunofluorescence. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX Camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.
Immunofluorescence Microscopy - HDAC Antibody. Histone deacetylase (HDAC) antibody detects HDAC (colored GREEN) as used in STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-HDAC and detected with 0.2 ug/mL DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody. Also shown in this 2-color STED image is an anti-tubulin monoclonal antibody [MOUSE] (detected with ATTO 425 conjugated anti-MOUSE IgG [GOAT] (secondary antibody (colored RED).
Anti-HDAC-1 Antibody - Western Blot. Western blot of Affinity Purified anti-HDAC-1 antibody shows detection of a band at ~65 kD corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-HDAC-1 Antibody - Western Blot. p/n was used for Western blot of LNCaP prostate cancer cells. Lysate was loaded at 50 ug per lane and incubated with 1:1000 dilution of primary Ab (Lot. 16248). A band was detected at the expected molecular weight of 55 Kda. Personal communication Flavio Rizzolio, Temple University.
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