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Thrombopoietin (TPO) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2028520
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MBS2028520 is an antibody targeting TPO. Also known as Polyclonal Antibody to Thrombopoietin (TPO). Reactive in Human, Mouse. Host: Rabbit. More details

$205 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Thrombopoietin (TPO), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Thrombopoietin (TPO)
Product Gene Name
anti-TPO antibody
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-SPA PPACDL RVLSKLLRDS HVLHSRLSQC PEVHPLPTPV LLPAVDFSL G EWKTQMEETK AQDILGAVTL LLEGVMAARG QLGPTCLSSL LGQLSGQVRL LLGALQSLL G TQGRTTAHKD PNAIFLSFQH LLRGKVRFLM LVGGSTLCVR RAPPTTAVPS RTSLVLTLNE LPNRTSGLLE TNFTASARTT GSGLLKWQQG FRAKIPGLLN QTSRSLDQIP GYLNRIHEL L NGTRGLFPGP SRRTLGAPDI SSGTSDTGSL PPNLQPGYSP SPTHPPTGQY TLFPLPPTL P TPVVQLHPLL PDPSAPTPTP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
Affinity Chromatography
Concentration
1mg/ml (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against TPO. It has beenselected for its ability to recognize TPO in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: TPO (MBS2028520)
Immunogen: TPO (MBS2031758)
Matching Pairs
Unconjugated Antibody: TPO (MBS2028520)
APC-CY7 Conjugated Antibody: TPO (MBS2038574)
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Molecular Weight
33,604 Da
OMIM
L33410 mRNA
3D Structure
ModBase 3D Structure for P40225
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Fragment
TPO (Ser22~Gly349)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant TPO (Ser22~Gly349) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2038578
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-TPO antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-TPO antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-TPO antibody

Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/mL Rabbit Anti-Human TPO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-TPO Polyclonal Antibody – Cat# MBS2028520

Western Blot (WB) of anti-TPO antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-TPO Polyclonal Antibody, Western Blot (WB) – Cat# MBS2028520

Immunohistochemistry (IHC) of anti-TPO antibody

DAB staining on fromalin fixed paraffin-embedded Liver tissue)
Rabbit Anti-TPO Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2028520

Immunohistochemistry (IHC) of anti-TPO antibody

DAB staining on IHC-P; Samples: Human Liver Tissue)
Rabbit Anti-TPO Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2028520

Other Notes

Small volumes of anti-TPO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TPO. It may not necessarily be applicable to this product.

NCBI GI #
4507493
NCBI GeneID
7066
NCBI Accession #
NP_000451.1
UniProt Primary Accession #
P40225
UniProt Secondary Accession #
Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8
UniProt Related Accession #
P40225
GenBank Nucleotide #
NM_000460.3
NCBI Official Full Name
thrombopoietin isoform 1
NCBI Official Synonym Full Names
thrombopoietin
NCBI Official Symbol
THPO
NCBI Official Synonym Symbols
ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
UniProt Protein Name
Thrombopoietin
UniProt Synonym Protein Names
C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand
Protein Family
Thyroid peroxidase
UniProt Gene Name
THPO
UniProt Synonym Gene Names
MGDF; ML; MGDF
NCBI Protein Information
thrombopoietin

NCBI Summary for TPO

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]
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UniProt Comments for TPO

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: extracellular space

Molecular Function: cytokine activity; growth factor activity; hormone activity

Biological Process: cell proliferation; multicellular organism development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation

Disease: Thrombocythemia 1
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Research Articles on TPO

1. These studies demonstrate that biallelic loss-of-function mutations in THPO cause bone marrow failure, which is unresponsive to transplant due to a hematopoietic cell-extrinsic mechanism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TPO antibody

 Reference Product  PubMed Publications
 MPL antibody  >34 publications with TPO and MPL
 JAK2 antibody  >4 publications with TPO and JAK2
 STAT3 antibody  >1 publications with TPO and STAT3

Pathways associated with anti-TPO antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Platelet Aggregation (Plug Formation) Pathway antibodies  Platelet Aggregation (Plug Formation) Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram

Diseases associated with anti-TPO antibody

 Disease Name  Pubmed Publications
 Thrombocytosis Antibodies  >11 publications with TPO and Thrombocytosis
 Anemia Antibodies  >8 publications with TPO and Anemia
 Hemorrhage Antibodies  >3 publications with TPO and Hemorrhage
 Lung Neoplasms Antibodies  >3 publications with TPO and Lung Neoplasms
 Disease Models, Animal Antibodies  >3 publications with TPO and Disease Models, Animal
 Neurobehavioral Manifestations Antibodies  >2 publications with TPO and Neurobehavioral Manifestations
 Growth Disorders Antibodies  >2 publications with TPO and Growth Disorders
 Thrombocythemia, Essential Antibodies  >2 publications with TPO and Thrombocythemia, Essential
 Precancerous Conditions Antibodies  >1 publications with TPO and Precancerous Conditions
 Prenatal Injuries Antibodies  >1 publications with TPO and Prenatal Injuries

Organs/Tissues associated with anti-TPO antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >7 publications with TPO and Liver
 Connective Tissue Antibodies  >1 publications with TPO and Connective Tissue
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