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MTBP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241294

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, and MTBP, were recently discovered. The transcriptional activity of… More details

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Product Overview

Online Datasheet in PDF
Product Name
MTBP, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human MTBP
Product Gene Name
Product Synonym Names
Anti-MTBP Antibody (aa122-139) IHC-plus; MTBP; Mdm2-binding protein; MDM2BP; HMTBP; Human MTBP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
synthetic peptide (gavecfeeedsnsresls) corresponding to amino acids 122 to 139 of human MTBP, which differ from the mouse sequence by three amino acids ()
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
37,253 Da
OMIM
605927
3D Structure
ModBase 3D Structure for Q96DY7
Target Species
Human
Immunogen Description
Synthetic peptide from human MTBP.
Immunogen Type
Synthetic peptide
Immunogen
MTBP antibody was raised against synthetic peptide from human MTBP.
Antigen Modification
aa122-139
Applications Tested/Suitable for anti-MTBP antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-MTBP antibody
ICC, IHC-P (10 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-MTBP antibody

Anti-MTBP antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-MTBP Polyclonal Antibody – Cat# MBS241294

Immunohistochemistry (IHC) of anti-MTBP antibody

Anti-MTBP antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-MTBP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS241294

Immunofluorescence (IF) of anti-MTBP antibody

Immunofluorescence of MTBP in K562 cells with MTBP antibody at 20 ug/ml.
Rabbit Anti-MTBP Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS241294

Immunocytochemistry (ICC) of anti-MTBP antibody

Immunocytochemistry of MTBP in K562 cells with MTBP antibody at 5 ug/ml.
Rabbit Anti-MTBP Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS241294

Western Blot (WB) of anti-MTBP antibody

Western blot of MTBP expression in K562 whole cell lysates with MTBP antibody at 1 ug /ml.
Rabbit Anti-MTBP Polyclonal Antibody, Western Blot (WB) – Cat# MBS241294

Other Notes

Small volumes of anti-MTBP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MTBP purchase

MBS241294 is an antibody targeting MTBP. Also known as Rabbit Polyclonal to Human MTBP. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MTBP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mdm2-binding protein
NCBI Official Synonym Full Names
MDM2 binding protein
NCBI Official Symbol
MTBP
NCBI Official Synonym Symbols
MDM2BP
UniProt Protein Name
Mdm2-binding protein
Protein Family
UniProt Gene Name
MTBP
UniProt Synonym Gene Names
hMTBP
UniProt Entry Name
MTBP_HUMAN
NCBI Protein Information
mdm2-binding protein; MDM2 (mouse double minute 2)-binding protein, 104kD; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein binding protein, 104kDa

NCBI Summary for MTBP

This gene encodes a protein that interacts with the oncoprotein mouse double minute 2. The encoded protein regulates progression through the cell cycle and may be involved in tumor formation. [provided by RefSeq, Aug 2012]

UniProt Comments for MTBP

MTBP: Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells. May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation. Belongs to the MTBP family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: kinetochore; chromatin

Biological Process: negative regulation of cell proliferation; traversing start control point of mitotic cell cycle; negative regulation of mitosis; cell cycle arrest
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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