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CCR8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241419

MBS241419 is an antibody targeting CCR8. Also known as Rabbit Polyclonal to Human CCR8. Reactive in Human; Predicted: Monkey. Host: Rabbit. More details

$630 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CCR8, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human CCR8
Product Gene Name
anti-CCR8 antibody
Product Synonym Names
Anti-CCR8 Antibody (Cytoplasmic Domain) IHC-plus; CCR8; C-C chemokine receptor type 8; CC chemokine receptor CHEMR1; CC-CKR-8; Chemokine (C-C) receptor 8; CDw198 antigen; CKRL1; CMKBR8; Chemokine receptor-like 1; CMKBRL2; CKR-L1; GPRCY6; TER1; C-C CKR-8; CC chemokine receptor 8; Human CCR8
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Predicted: Monkey
Form/Format
PBS, 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human CCR8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
OMIM
601834
3D Structure
ModBase 3D Structure for P51685
Immunogen Species
CCR8 antibody was raised against Human
Antigen Type
Synthetic peptide
Immunogen
CCR8 antibody was raised against synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human CCR8. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon, Marmoset (95%); Bovine, Horse (84%).
Epitope
Cytoplasmic Domain
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CCR8 antibody

Family: GPCR; Subfamily: Chemokine
Applications Tested/Suitable for anti-CCR8 antibody
Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-Paraffin), ELISA (EIA)
Application Notes for anti-CCR8 antibody
IHC
IHC-Paraffin (5 ug/ml)
ELISA
(optimal dilution to be determined by the researcher)

Immunohistochemistry: MBS241419 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241419 was determined to be 5 ug/ml.

Other Notes

Small volumes of anti-CCR8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CCR8. It may not necessarily be applicable to this product.

NCBI GI #
4885121
NCBI GeneID
1237
NCBI Accession #
NP_005192.1
UniProt Primary Accession #
P51685
UniProt Secondary Accession #
Q3KNQ8; Q3KNR3; Q9BYX5; B2RC64
UniProt Related Accession #
P51685
GenBank Nucleotide #
NM_005201.3
NCBI Official Full Name
C-C chemokine receptor type 8
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 8
NCBI Official Symbol
CCR8
NCBI Official Synonym Symbols
CY6; TER1; CCR-8; CKRL1; CDw198; CMKBR8; GPRCY6; CMKBRL2; CC-CKR-8
UniProt Protein Name
C-C chemokine receptor type 8
UniProt Synonym Protein Names
CC chemokine receptor CHEMR1; CMKBRL2; Chemokine receptor-like 1; CKR-L1; GPR-CY6; GPRCY6; TER1; CD_antigen: CDw198
Protein Family
C-C chemokine receptor
UniProt Gene Name
CCR8
UniProt Synonym Gene Names
CKRL1; CMKBR8; CMKBRL2; C-C CKR-8; CC-CKR-8; CCR-8; CKR-L1; GPRCY6
UniProt Entry Name
CCR8_HUMAN
NCBI Protein Information
C-C chemokine receptor type 8; CC chemokine receptor 8; chemokine receptor-like 1; chemokine (C-C) receptor 8; CC chemokine receptor CHEMR1; CC-chemokine receptor chemr1; chemokine (C-C) receptor-like 2

NCBI Summary for CCR8

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are important for the migration of various cell types into the inflammatory sites. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP-1 beta) have been identified as ligands of this receptor. Studies of this receptor and its ligands suggested its role in regulation of monocyte chemotaxis and thymic cell apoptosis. More specifically, this receptor may contribute to the proper positioning of activated T cells within the antigenic challenge sites and specialized areas of lymphoid tissues. This gene is located at the chemokine receptor gene cluster region. [provided by RefSeq, Jul 2008]
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UniProt Comments for CCR8

CCR8: Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; Motility/polarity/chemotaxis; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: C-C chemokine receptor activity; chemokine receptor activity; coreceptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; immune response; chemotaxis; cell adhesion
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Research Articles on CCR8

1. CCL1-CCR8 interaction may play a critical role in lymphocytic recruitment in IgG4-related sclerosing cholangitis and type 1 autoimmune pancreatitis, leading to duct-centred inflammation and obliterative phlebitis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CCR8 antibody

 Reference Product  PubMed Publications
 CCL1 antibody  >101 publications with CCR8 and CCL1
 CCL17 antibody  >34 publications with CCR8 and CCL17
 CCL5 antibody  >24 publications with CCR8 and CCL5
 CCL4 antibody  >24 publications with CCR8 and CCL4
 CXCL10 antibody  >15 publications with CCR8 and CXCL10
 CXCL9 antibody  >8 publications with CCR8 and CXCL9
 CCL16 antibody  >8 publications with CCR8 and CCL16
 CCL20 antibody  >7 publications with CCR8 and CCL20
 CXCL11 antibody  >4 publications with CCR8 and CXCL11
 CCL21 antibody  >1 publications with CCR8 and CCL21

Pathways associated with anti-CCR8 antibody

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram

Diseases associated with anti-CCR8 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >111 publications with CCR8 and Immune System Diseases
 Inflammation Antibodies  >75 publications with CCR8 and Inflammation
 Respiratory Tract Diseases Antibodies  >38 publications with CCR8 and Respiratory Tract Diseases
 Lung Diseases Antibodies  >36 publications with CCR8 and Lung Diseases
 Disease Models, Animal Antibodies  >27 publications with CCR8 and Disease Models, Animal
 Autoimmune Diseases Antibodies  >22 publications with CCR8 and Autoimmune Diseases
 Necrosis Antibodies  >17 publications with CCR8 and Necrosis
 Encephalomyelitis, Autoimmune, Experimental Antibodies  >6 publications with CCR8 and Encephalomyelitis, Autoimmune, Experimental
 Hypersensitivity, Delayed Antibodies  >5 publications with CCR8 and Hypersensitivity, Delayed
 Kidney Diseases Antibodies  >3 publications with CCR8 and Kidney Diseases

Organs/Tissues associated with anti-CCR8 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >189 publications with CCR8 and Blood
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