Goat Anti-NET1 Polyclonal Antibody – Cat# MBS241645

Anti-NET1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

NET1 Antibody – Goat Polyclonal

Name: NET1 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS241645
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241645

MBS241645 is an antibody targeting NET1. Also known as Goat Polyclonal to Human NET1. Reactive in Gorilla, HumanPredicted Reactivity: Orangutan, Gibbon, Monkey (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

NET1, Polyclonal Antibody

Full Product Name

Goat Polyclonal to Human NET1

Product Gene Name

anti-NET1 antibody

Product Synonym Names

Anti-NET1 Antibody (C-Terminus) IHC-plus; NET1; ARHGEF8; NET1A; Proto-oncogene p65 Net1; Human NET1

Clonality

Polyclonal

Host

Goat

Species Reactivity

Gorilla, Human
Predicted Reactivity: Orangutan, Gibbon, Monkey (at least 90% immunogen sequence identity)

Form/Format

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

Purity/Purification

Immunoaffinity Purified

Concentration

0.5 mg/ml (lot specific)

Specificity

Human NET1. This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).

Preparation and Storage

Store at -20 degree C. Minimize freezing and thawing.

Molecular Weight

61,885 Da

OMIM

606450

3D Structure

ModBase 3D Structure for Q7Z628

Target Species

Human

Immunogen Description

Synthetic peptide C-DKALSGGKRKETLV from the C-terminus of human NET1 (NP_001040625.1; NP_005854.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Orangutan, Gibbon, Monkey (93%); Marmoset, Mouse, Hamster (86%).

Immunogen Type

Synthetic peptide

Immunogen

NET1 antibody was raised against synthetic peptide C-DKALSGGKRKETLV from the C-terminus of human NET1 (NP_001040625.1; NP_005854.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Orangutan, Gibbon, Monkey (93%); Marmoset, Mouse, Hamster (86%).

Antigen Modification

C-Terminus

Applications Tested/Suitable for anti-NET1 antibody

Immunohistochemistry (IHC - Paraffin), ELISA (EIA)

Application Notes for anti-NET1 antibody

ELISA (1:32000), IHC-P (2.5 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Other Notes

Small volumes of anti-NET1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NET1. It may not necessarily be applicable to this product.

NCBI GI #

19923327

NCBI GeneID

10276

NCBI Accession #

NP_005854.2

UniProt Primary Accession #

Q7Z628

UniProt Secondary Accession #

Q12773; Q96D82; Q99903; Q9UEN6

UniProt Related Accession #

Q7Z628

GenBank Nucleotide #

NM_005863.4

NCBI Official Full Name

neuroepithelial cell-transforming gene 1 protein isoform 2

NCBI Official Synonym Full Names

neuroepithelial cell transforming 1

NCBI Official Symbol

NET1

NCBI Official Synonym Symbols

NET1A; ARHGEF8

UniProt Protein Name

Neuroepithelial cell-transforming gene 1 protein

UniProt Synonym Protein Names

Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8

Protein Family

Neuroepithelial cell-transforming gene 1 protein

UniProt Gene Name

NET1

UniProt Synonym Gene Names

ARHGEF8

UniProt Entry Name

ARHG8_HUMAN

NCBI Protein Information

neuroepithelial cell-transforming gene 1 protein; p65 Net1 proto-oncogene protein; small GTP-binding protein regulator; neuroepithelioma transforming gene 1; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene)

NCBI Summary for NET1

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

UniProt Comments for NET1

NET1: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: plasma membrane; cytosol; nucleus

Molecular Function: Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; GTP-Rho binding

Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; myoblast migration; regulation of cell growth; signal transduction

Research Articles on NET1

1. NET1 expression is elevated in OAC and its pre-malignant phenotype, Barrett's oesophagus. NET1 promotes OAC cell invasion and proliferation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NET1 antibody

Reference Product	PubMed Publications
RHOA antibody	>24 publications with NET1 and RHOA
DLG1 antibody	>2 publications with NET1 and DLG1
MAGI1 antibody	>2 publications with NET1 and MAGI1

Pathways associated with anti-NET1 antibody

Products by Pathway	Pathway Diagram
Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies	Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
G Alpha (12/13) Signalling Events Pathway antibodies	G Alpha (12/13) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
NRAGE Signals Death Through JNK Pathway antibodies	NRAGE Signals Death Through JNK Pathway Diagram
Regulation Of RhoA Activity Pathway antibodies	Regulation Of RhoA Activity Pathway Diagram
Rho GTPase Cycle Pathway antibodies	Rho GTPase Cycle Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram
Signaling By Rho GTPases Pathway antibodies	Signaling By Rho GTPases Pathway Diagram
Signalling By NGF Pathway antibodies	Signalling By NGF Pathway Diagram

Diseases associated with anti-NET1 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>14 publications with NET1 and Adenocarcinoma
Breast Neoplasms Antibodies	>12 publications with NET1 and Breast Neoplasms
Liver Diseases Antibodies	>9 publications with NET1 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>8 publications with NET1 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>8 publications with NET1 and Liver Neoplasms
Brain Diseases Antibodies	>4 publications with NET1 and Brain Diseases
Neoplasms, Experimental Antibodies	>3 publications with NET1 and Neoplasms, Experimental
Cell Transformation, Neoplastic Antibodies	>2 publications with NET1 and Cell Transformation, Neoplastic
Disease Models, Animal Antibodies	>2 publications with NET1 and Disease Models, Animal
Hypertension Antibodies	>1 publications with NET1 and Hypertension

Organs/Tissues associated with anti-NET1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>10 publications with NET1 and Brain
Liver Antibodies	>9 publications with NET1 and Liver
Blood Antibodies	>7 publications with NET1 and Blood
Nerve Antibodies	>5 publications with NET1 and Nerve
Stomach Antibodies	>4 publications with NET1 and Stomach
Embryonic Tissue Antibodies	>4 publications with NET1 and Embryonic Tissue
Vascular Antibodies	>3 publications with NET1 and Vascular
Esophagus Antibodies	>2 publications with NET1 and Esophagus
Eye Antibodies	>2 publications with NET1 and Eye
Muscle Antibodies	>2 publications with NET1 and Muscle