• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

RNF174 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241687

MBS241687 is an antibody targeting RNF174. Also known as Rabbit Polyclonal to Human RNF174 / MARCH4. Reactive in Chimpanzee, Chicken, Dog, Horse, Human, Mouse, Opossum, Rat, Zebrafish. Host: Rabbit. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
RNF174 / MARCH4, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human RNF174 / MARCH4
Product Gene Name
Product Synonym Gene Name
MARCH4
Product Synonym Names
Anti-RNF174 / MARCH4 Antibody (aa300-350) IHC-plus; MARCH4; RING finger protein 174; KIAA1399; MARCH-IV; RNF174; Human RNF174; MARCH4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Chimpanzee, Chicken, Dog, Horse, Human, Mouse, Opossum, Rat, Zebrafish
Form/Format
PBS, 0.2% gelatin, 0.05% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
A portion of amino acids 300-350 of human RNF174
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
45,528 Da
OMIM
608208
3D Structure
ModBase 3D Structure for Q9P2E8
Target Species
Human
Immunogen Description
Synthetic peptide from human MARCH4.
Immunogen Type
Synthetic peptide
Immunogen
RNF174 / MARCH4 antibody was raised against synthetic peptide from human MARCH4.
Antigen Modification
aa300-350
Applications Tested/Suitable for anti-RNF174 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-RNF174 antibody
IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-RNF174 antibody

Anti-MARCH4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-RNF174 Polyclonal Antibody – Cat# MBS241687

Western Blot (WB) of anti-RNF174 antibody

Western blot of human RNF174 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 0.5 ug/ml.
Rabbit Anti-RNF174 Polyclonal Antibody, Western Blot (WB) – Cat# MBS241687

Other Notes

Small volumes of anti-RNF174 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RNF174. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH4
NCBI Official Synonym Full Names
membrane-associated ring finger (C3HC4) 4, E3 ubiquitin protein ligase
NCBI Official Symbol
MARCH4
NCBI Official Synonym Symbols
RNF174; MARCH-IV
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH4
UniProt Synonym Protein Names
Membrane-associated RING finger protein 4; Membrane-associated RING-CH protein IV; MARCH-IV; RING finger protein 174
UniProt Gene Name
MARCH4
UniProt Synonym Gene Names
KIAA1399; RNF174; MARCH-IV
UniProt Entry Name
MARH4_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH4; RING finger protein 174; membrane-associated RING-CH protein IV; membrane-associated RING finger protein 4

NCBI Summary for RNF174

MARCH4 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH4 reduces surface accumulation of several membrane glycoproteins by directing them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]
Show more

UniProt Comments for RNF174

MARCH4: E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Membrane protein, multi-pass; Ubiquitin ligase; EC 6.3.2.-; Ligase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: Golgi membrane; Golgi stack; integral to membrane; trans-Golgi network

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein ubiquitination
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-RNF174 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1 publications with RNF174 and Liver
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000