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HTR1D Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241844

MBS241844 is an antibody targeting HTR1D. Also known as Rabbit Polyclonal (IgG) to Rat HTR1D / 5-HT1D Receptor. Reactive in Human, Mouse, Rat. Host: Rabbit. The 5-HT1D receptor is a member of the Serotonin Receptor subfamily. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
HTR1D / 5-HT1D Receptor, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Rat HTR1D / 5-HT1D Receptor
Product Gene Name
anti-HTR1D antibody
Product Synonym Names
Anti-HTR1D / 5-HT1D Receptor Antibody (aa1-18) IHC-plus; HTR1D; 5-HT1d alpha receptor; 5HT1D Receptor; 5-HT1d-type serotonin receptor; 5-HT1D; 5-HT-1D; 5-HT-1D-alpha; 5-HT1d receptor; HT1d receptor; HTR1DA; HTRL; HT1DA; Serotonin receptor 1D; RDC4; Gpcr14; Serotonin 1d receptor; Serotonin 5-HT-1d receptor; Rat HTR1D; 5-HT1D Receptor
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide, 0.05% BSA.
Purity/Purification
Protein G Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
A mixture of synthetic peptides corresponding to amino acids 1-18 and 251-267 of rat 5-HT1DR.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Immunogen Sequence Positions
1-18
OMIM
182133
3D Structure
ModBase 3D Structure for P28221
Target
HTR1D/5-HT1D Receptor
Target Species
Rat
Immunogen Description
Synthetic peptide from rat HTR1D/5-HT1D.
Immunogen Type
Synthetic peptide
Immunogen
HTR1D / 5-HT1D Receptor antibody was raised against synthetic peptide from rat HTR1D / 5-HT1D.
Antigen Modification
aa1-18
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-HTR1D antibody

Family: GPCR; Subfamily: Serotonin
Applications Tested/Suitable for anti-HTR1D antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-HTR1D antibody
IHC-P (10 ug/ml), WB (2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary anitbody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal woking concentration MBS241844 was determined to be 10ug/ml.

Immunohistochemistry (IHC) of anti-HTR1D antibody

Anti-5HT1D Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-HTR1D Polyclonal Antibody – Cat# MBS241844

Western Blot (WB) of anti-HTR1D antibody

Western blot of 5-HT1DR in brain lysate with anti-5-HT1DR pcAb. A protein band of approximate molecular weight of 40 kDa was detected.
Rabbit Anti-HTR1D Polyclonal Antibody, Western Blot (WB) – Cat# MBS241844

Other Notes

Small volumes of anti-HTR1D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HTR1D. It may not necessarily be applicable to this product.

NCBI GI #
4504535
NCBI GeneID
3352
NCBI Accession #
NP_000855.1
UniProt Primary Accession #
P28221
UniProt Related Accession #
P28221
GenBank Nucleotide #
NM_000864.4
NCBI Official Full Name
5-hydroxytryptamine receptor 1D
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 1D, G protein-coupled
NCBI Official Symbol
HTR1D
NCBI Official Synonym Symbols
HTRL; RDC4; HT1DA; 5-HT1D; HTR1DA
UniProt Protein Name
5-hydroxytryptamine receptor 1D
UniProt Synonym Protein Names
Serotonin 1D alpha receptor; 5-HT-1D-alpha; Serotonin receptor 1D
Protein Family
5-hydroxytryptamine receptor
UniProt Gene Name
HTR1D
UniProt Synonym Gene Names
HTR1DA; HTRL; 5-HT-1D; 5-HT1D; 5-HT-1D-alpha
UniProt Entry Name
5HT1D_HUMAN
NCBI Protein Information
5-hydroxytryptamine receptor 1D; 5-HT-1D; 5-HT-1D-alpha; serotonin receptor 1D; serotonin 1D alpha receptor

UniProt Comments for HTR1D

5-HT(1D): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.3-p34.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: serotonin binding; serotonin receptor activity

Biological Process: regulation of locomotion; G-protein signaling, coupled to cyclic nucleotide second messenger; vasoconstriction; synaptic transmission; intestine smooth muscle contraction; response to toxin; G-protein signaling, adenylate cyclase inhibiting pathway; serotonin receptor signaling pathway; regulation of behavior
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Research Articles on HTR1D

1. study identified the presence of genetic association at chromosome 1p36 with migraine with aura (P=0.045, Bonferroni corrected): the locus encoding the 5HT(1D) receptor gene

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HTR1D antibody

 Reference Product  PubMed Publications
 HTR1B antibody  >9 publications with HTR1D and HTR1B
 HTR1A antibody  >9 publications with HTR1D and HTR1A
 OPRD1 antibody  >3 publications with HTR1D and OPRD1
 DRD4 antibody  >1 publications with HTR1D and DRD4

Pathways associated with anti-HTR1D antibody

 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Metabolic Disorders Of Biological Oxidation Enzymes Pathway antibodies  Metabolic Disorders Of Biological Oxidation Enzymes Pathway Diagram
 Monoamine GPCRs Pathway antibodies  Monoamine GPCRs Pathway Diagram

Diseases associated with anti-HTR1D antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >15 publications with HTR1D and Mental Disorders
 Nervous System Diseases Antibodies  >2 publications with HTR1D and Nervous System Diseases
 Learning Disorders Antibodies  >1 publications with HTR1D and Learning Disorders
 Weight Gain Antibodies  >1 publications with HTR1D and Weight Gain
 Depressive Disorder Antibodies  >1 publications with HTR1D and Depressive Disorder
 Vomiting Antibodies  >1 publications with HTR1D and Vomiting
 Movement Disorders Antibodies  >1 publications with HTR1D and Movement Disorders
 Weight Loss Antibodies  >1 publications with HTR1D and Weight Loss
 Autistic Disorder Antibodies  >1 publications with HTR1D and Autistic Disorder

Organs/Tissues associated with anti-HTR1D antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with HTR1D and Brain
 Intestine Antibodies  >3 publications with HTR1D and Intestine
 Eye Antibodies  >1 publications with HTR1D and Eye
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