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SAE2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241878

MBS241878 is an antibody targeting SAE2. Also known as Rabbit Polyclonal (IgG) to Human SAE2 / UBA2. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SAE2 / UBA2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human SAE2 / UBA2
Product Gene Name
Product Synonym Gene Name
UBA2
Product Synonym Names
Anti-SAE2 / UBA2 Antibody (aa160-210) IHC-plus; UBA2; SAE2; UBLE1B; HRIHFB2115; Human SAE2; UBA2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.2% gelatin, 0.05% sodium azide.
Purity/Purification
Protein G Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
A synthetic peptide within amino acid residues 160-210 of human SAE2 was used as the immunogen.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
60,839 Da
OMIM
613295
3D Structure
ModBase 3D Structure for Q9UBT2
Target Species
Human
Immunogen Description
Synthetic peptide from human Uba2.
Immunogen Type
Synthetic peptide
Immunogen
SAE2 / UBA2 antibody was raised against synthetic peptide from human Uba2.
Antigen Modification
aa160-210
Applications Tested/Suitable for anti-SAE2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-SAE2 antibody
IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-SAE2 antibody

Anti-UBA2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-SAE2 Polyclonal Antibody – Cat# MBS241878

Western Blot (WB) of anti-SAE2 antibody

Western blot of SAE2 in HeLa cell lysate using antibody at 1.0 ug/ml.
Rabbit Anti-SAE2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS241878

Other Notes

Small volumes of anti-SAE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SAE2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
SUMO-activating enzyme subunit 2
NCBI Official Synonym Full Names
ubiquitin-like modifier activating enzyme 2
NCBI Official Symbol
UBA2
NCBI Official Synonym Symbols
ARX; SAE2; HRIHFB2115
UniProt Protein Name
SUMO-activating enzyme subunit 2
UniProt Synonym Protein Names
Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2
Protein Family
UniProt Gene Name
UBA2
UniProt Synonym Gene Names
SAE2; UBLE1B
UniProt Entry Name
SAE2_HUMAN
NCBI Protein Information
SUMO-activating enzyme subunit 2; SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; ubiquitin-like 1-activating enzyme E1B; anthracycline-associated resistance ARX; ubiquitin-like modifier-activating enzyme 2; UBA2, ubiquitin-activating enzyme E1 homolog

NCBI Summary for SAE2

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
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UniProt Comments for SAE2

SAE2: a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.

Protein type: Motility/polarity/chemotaxis; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: SUMO activating enzyme activity; protein binding; protein heterodimerization activity; metal ion binding; enzyme activator activity; transcription factor binding; ATP binding

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; SMT3-dependent protein catabolic process; post-translational protein modification
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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