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CENTB1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241950

MBS241950 is an antibody targeting CENTB1. Also known as Goat Polyclonal to Human CENTB1 / ACAP1. Reactive in Gibbon, Bovine, Dog, Gorilla, Human, Monkey, Mouse, Rabbit, RatPredicted Reactivity: Horse, Pig (at least 90% immunogen sequence identity). Host:… More details

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Testing Data View PDF
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Product Overview

Product Name
CENTB1 / ACAP1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human CENTB1 / ACAP1
Product Gene Name
anti-CENTB1 antibody
Product Synonym Gene Name
ACAP1
Product Synonym Names
Anti-CENTB1 / ACAP1 Antibody (C-Terminus) IHC-plus; ACAP1; Centaurin; beta 1; Centaurin-beta-1; Cnt-b1; KIAA0050; CENTB1; Human CENTB1; ACAP1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Bovine, Dog, Gorilla, Human, Monkey, Mouse, Rabbit, Rat
Predicted Reactivity: Horse, Pig (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human ACAP1 / CENTB1.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
81,536 Da
OMIM
607763
3D Structure
ModBase 3D Structure for Q15027
Target Species
Human
Immunogen Description
Synthetic peptide C-EKLSRRSHDLHTL from the C-terminus of human ACAP1 / CENTB1 (NP_055531.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Elephant, Panda, Rabbit, Opossum (100%).
Immunogen Type
Synthetic peptide
Immunogen
CENTB1 / ACAP1 antibody was raised against synthetic peptide C-EKLSRRSHDLHTL from the C-terminus of human ACAP1 / CENTB1 (NP_055531.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Elephant, Panda, Rabbit, Opossum (100%); Horse, Pig (92%); Bat (85%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-CENTB1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CENTB1 antibody
ELISA (1:32000), IHC-P (5 ug/ml), WB (1:32000, 0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-CENTB1 antibody

Anti-ACAP1 / CENTB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-CENTB1 Polyclonal Antibody – Cat# MBS241950

Western Blot (WB) of anti-CENTB1 antibody

Antibody2 staining (0.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Goat Anti-CENTB1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS241950

Other Notes

Small volumes of anti-CENTB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CENTB1. It may not necessarily be applicable to this product.

NCBI GI #
7661880
NCBI GeneID
9744
NCBI Accession #
NP_055531.1
UniProt Primary Accession #
Q15027
UniProt Secondary Accession #
Q53XN9
UniProt Related Accession #
Q15027
GenBank Nucleotide #
NM_014716.3
NCBI Official Full Name
arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
NCBI Official Synonym Full Names
ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
NCBI Official Symbol
ACAP1
NCBI Official Synonym Symbols
CENTB1
UniProt Protein Name
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
UniProt Synonym Protein Names
Centaurin-beta-1; Cnt-b1
UniProt Gene Name
ACAP1
UniProt Synonym Gene Names
CENTB1; KIAA0050; Cnt-b1
UniProt Entry Name
ACAP1_HUMAN
NCBI Protein Information
arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; cnt-b1; centaurin-beta-1; centaurin, beta 1; Arf GAP with coiled coil, ANK repeat and PH domains 1

UniProt Comments for CENTB1

CENTB1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.

Protein type: Motility/polarity/chemotaxis; GAPs, ARF; GAPs

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; recycling endosome membrane

Molecular Function: zinc ion binding

Biological Process: protein transport; positive regulation of GTPase activity
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Research Articles on CENTB1

1. ACAP1 and ARAP2 each colocalize with Arf6 but they did not colocalize with each other and have opposing effects on focal adhesions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CENTB1 antibody

 Reference Product  PubMed Publications
 ARF6 antibody  >13 publications with CENTB1 and ARF6
 ARF5 antibody  >1 publications with CENTB1 and ARF5

Pathways associated with anti-CENTB1 antibody

 Products by Pathway  Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 NOD Pathway antibodies  NOD Pathway Diagram

Diseases associated with anti-CENTB1 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >1 publications with CENTB1 and Disease Models, Animal
 Necrosis Antibodies  >1 publications with CENTB1 and Necrosis

Organs/Tissues associated with anti-CENTB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with CENTB1 and Brain
 Lung Antibodies  >2 publications with CENTB1 and Lung
 Nerve Antibodies  >1 publications with CENTB1 and Nerve
 Intestine Antibodies  >1 publications with CENTB1 and Intestine
 Muscle Antibodies  >1 publications with CENTB1 and Muscle
 Heart Antibodies  >1 publications with CENTB1 and Heart
 Vascular Antibodies  >1 publications with CENTB1 and Vascular
 Blood Antibodies  >1 publications with CENTB1 and Blood
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